EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of h and m antigens of histoplasma capsulatum free of heterologous antigens


, : Preparation of h and m antigens of histoplasma capsulatum free of heterologous antigens. Current Microbiology 12(4): 209-216

Salt gradient elution of crude histoplasmin on CM-Sepharose CL6B at pH 3.0 was used in essentially a one-step procedure to isolate the h, m, and non-m antigens of Histoplasma capsulatum and free them of any c antigen common to other pathogenic fungi. The h antigen fraction was pure; the isolated m and non-m antigens contained less than 0.1% of the c antigen present in the original preparation. The residual c antigen in these fractions was removed by affinity chromatography on Affigel-10 coupled to a Blastomyces dermatitidis fungal antiserum. The final preparations of h, m, and non-m were pure when tested by immunodiffusion (ID), sodium-dodecyl-sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), or electrofocusing. The antigens were serologically stable at neutral pH for at least three months at 10.degree. C. The relative molecular weights, isoelectric points, and amino acid composition of the antigens are described.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006173192

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Klein, T. D.; Encinias, A. M.; Encinias, H. B.; Bauer, M. L.; Caton, J. S.; Lardy, G. P., 2001: Energy supplementation of nursing beef calves on native range in southeastern North Dakota. Journal of Animal Science 79(Supplement 2): 108-109

Kanke, Minoru, 1996: Total laryngectomy to prevent aspiration: Report of four cases. Total laryngectomy, although it produces the most reliable effects in a short period to the patients with neural disorders, was not often been performed because of permanent loss of voice and speech, and of psychological damage due to loss of the...

Sang, N. N.; Tra, L. N.; Phi, P. T. P., 1999: Immune status of children suffering from idiopathic nephrotic syndrome. Pediatric Nephrology 13(7): C60, Sept

Polsky B.; Wolitz R.; Cantrill H.; Chuang E.L.; Heinemann M H.; Sands M.; Feinberg J.E.; Power M.; Davis R., 1991: Intravitreal ivl ganciclovir gcv salvage therapy rx for cytomegalovirus cmv retinitis actg 085 a preliminary report. Istituto Superiore Di Sanita Vii International Conference on Aids: Science Challenging Aids; Florence, Italy, June 16-21, 464p (Vol 1); 460p (Vol 2) Istituto Superiore Di Sanita: Rome, Italy Paper : 267b

Cerutti P.A., 1978: Repairable damage in dna. Journal of Supramolecular Structure 7(SUPPL 2):

Aanonsen, A., 1973: Constructing an automatic shade for greenhouses. Gartneryket 63 (36) 632-633

Indravadan R. Kothari; Gregory C. Martin; Peter J. Reilly; Peter J. Martin; James M. Eakman, 1972: Estimation of parameters in population models for schizosaccharomyces pombe from chemostat data. Biotechnology and Bioengineering 14(6): 915-938

Wang Bin; Xue Wujun; Feng Xinshun, 2000: The analysis of the factors inducing HLA sensitization in first renal-transplant recipients. Objective To study the relationship between multi-transfusion, multi-pregnancy and sensitization. Methods Tested the anti-HLA IgG in the serum of patients by ELISA. Results The rates of sensitization in patients with the history of multi-transfusi...

Orlandella, V.; Squeri, L., 1958: Electron microscopy of Salmonella pullorum and S. gallinarum. The technique adopted is described. There was no morphological difference between the two organisms, which were highly pleomorphic and varied in size. After incubation for a few hours globular forms appeared. Other forms undergoing lysis could als...

Bellet, Samuel; Muller, Otto F.; Herring, Allen B.; La Van, Donaldw, 1963: Effect of erythrityl tetranitrate on the electrocardiogram as recorded during exercise by radioelectrocardiography. The effect of erythrityl tetranitrate administered sublingually in a dose of 15 mg was evaluated in 43 patients with angina pectoris by means of radioelectrocardiography. Leads II, V4 and V6 were telemetered successively for 10 seconds each throug...