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Preparation of h and m antigens of histoplasma capsulatum free of heterologous antigens


Current Microbiology 12(4): 209-216
Preparation of h and m antigens of histoplasma capsulatum free of heterologous antigens
Salt gradient elution of crude histoplasmin on CM-Sepharose CL6B at pH 3.0 was used in essentially a one-step procedure to isolate the h, m, and non-m antigens of Histoplasma capsulatum and free them of any c antigen common to other pathogenic fungi. The h antigen fraction was pure; the isolated m and non-m antigens contained less than 0.1% of the c antigen present in the original preparation. The residual c antigen in these fractions was removed by affinity chromatography on Affigel-10 coupled to a Blastomyces dermatitidis fungal antiserum. The final preparations of h, m, and non-m were pure when tested by immunodiffusion (ID), sodium-dodecyl-sulfate-polyacrylamide-gel electrophoresis (SDS-PAGE), or electrofocusing. The antigens were serologically stable at neutral pH for at least three months at 10.degree. C. The relative molecular weights, isoelectric points, and amino acid composition of the antigens are described.


Accession: 006173192



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