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Preparation of haptens for use in immunoassays of tetra hydro 11 deoxy cortisol and its glucuronides


, : Preparation of haptens for use in immunoassays of tetra hydro 11 deoxy cortisol and its glucuronides. Chemical & Pharmaceutical Bulletin (Tokyo) 31(11): 4001-4007

For developing immunoassays of tetrahydro-11-deoxycortisol or its glucuronides, various haptenic derivatives were synthesized. The 3-hemisuccinate (15), 21-hemisuccinate (8), 3-hemiglutarate (16), 21-hemiglutarate (9) and glucuronides of tetrahydro-11-deoxycortisol were prepared starting from 11-deoxycortisol 21-acetate (1). Antisera were elicited in 2 rabbits by immunization with the conjugate of tetrahydro-11-deoxycortisol 3-glucuronide (19) with bovine serum albumin. The antisera had binding affinities to enzyme-labeled antigens prepared from 15, 16 and 19; the binding was inhibited by the glucuronide (19) in the enzyme immunoassay procedure.

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Accession: 006173194

PMID: 6671239

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