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Preparation of haptens for use in immunoassays of tetra hydro 11 deoxy cortisol and its glucuronides


, : Preparation of haptens for use in immunoassays of tetra hydro 11 deoxy cortisol and its glucuronides. Chemical & Pharmaceutical Bulletin (Tokyo) 31(11): 4001-4007

For developing immunoassays of tetrahydro-11-deoxycortisol or its glucuronides, various haptenic derivatives were synthesized. The 3-hemisuccinate (15), 21-hemisuccinate (8), 3-hemiglutarate (16), 21-hemiglutarate (9) and glucuronides of tetrahydro-11-deoxycortisol were prepared starting from 11-deoxycortisol 21-acetate (1). Antisera were elicited in 2 rabbits by immunization with the conjugate of tetrahydro-11-deoxycortisol 3-glucuronide (19) with bovine serum albumin. The antisera had binding affinities to enzyme-labeled antigens prepared from 15, 16 and 19; the binding was inhibited by the glucuronide (19) in the enzyme immunoassay procedure.


Accession: 006173194

PMID: 6671239

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Vielhauer W.; Mok M.; Mittelstaedt G.V.; Gless K.H.; Vecsei P., 1976: Estimation of tetra hydro 11 deoxy cortisol and tetra hydro 11 deoxy cortisol glucuronide equivalents after the metyrapone test by radio immunoassay and comparison with plasma 11 deoxy cortisol concentrations. Acta Endocrinologica Supplementum 202: 74-75

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