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Preparation of high density lipo proteins labeled exclusively at the cholesteryl ester moiety

Clinica Chimica Acta 123(1-2): 139-144

Preparation of high density lipo proteins labeled exclusively at the cholesteryl ester moiety

The in vitro preparation of [human] HDL [high density lipoprotein] radiolabeled at the cholesteryl ester moiety was determined by incubating different serum fractions with [3H]cholesteryl linoleate or cholesteryl [14C]oleate dissolved in dimethylsulfoxide (DMSO) and subsequent reisolation of HDL from the incubation mixture by ultracentrifugation. Using the supernatant obtained by precipitation of apolipoprotein B-containing lipoproteins with phosphotungstate/MgCl2 or using the > 1.075 kg/l fraction of sera, the bulk of the added radioactivity could be recovered in the reisolated HDL fraction, as demonstrated by density gradient ultracentrifugation or by gel chromatography. Incubation experiments using whole sera or the > 1.063 kg/l fraction of sera resulted in the preferential association of the labeled cholesteryl linoleate with apolipoprotein B-containing lipoproteins.

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Accession: 006173210

PMID: 7116635

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