EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of high density lipo proteins labeled exclusively at the cholesteryl ester moiety


, : Preparation of high density lipo proteins labeled exclusively at the cholesteryl ester moiety. Clinica Chimica Acta 123(1-2): 139-144

The in vitro preparation of [human] HDL [high density lipoprotein] radiolabeled at the cholesteryl ester moiety was determined by incubating different serum fractions with [3H]cholesteryl linoleate or cholesteryl [14C]oleate dissolved in dimethylsulfoxide (DMSO) and subsequent reisolation of HDL from the incubation mixture by ultracentrifugation. Using the supernatant obtained by precipitation of apolipoprotein B-containing lipoproteins with phosphotungstate/MgCl2 or using the > 1.075 kg/l fraction of sera, the bulk of the added radioactivity could be recovered in the reisolated HDL fraction, as demonstrated by density gradient ultracentrifugation or by gel chromatography. Incubation experiments using whole sera or the > 1.063 kg/l fraction of sera resulted in the preferential association of the labeled cholesteryl linoleate with apolipoprotein B-containing lipoproteins.

(PDF 0-2 workdays service)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006173210

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Pelster, Bernd., 1997: Buoyancy at depth. Unknown

Anonymous, 2004: Managing grief responses. DVM 35(10): 4E

Kim, H. , R.; Kim, H. , Y.; Lee, S. , H., 2006: Elevated serum levels of soluble receptor activator of nuclear factors-kappa B ligand (sRANKL) and reduced bone mineral density in patients with ankylosing spondylitis (AS). Objective. To assess bone mineral density (BMD), serum levels of soluble receptor activator of nuclear factors-kappa B ligand (sRANKL) and osteoprotegerin (OPG) in patients with ankylosing spondylitis (AS), and to determine their relationships wit...

Fallowfield, L., 2002: Talking about difficult things in cancer Do we achieve what patients need?. Drugs Of Today. 38(suppl. A): 79-87

Chekin S. S., 1992: Preparation of stable suspensions of delaminated kaolinite by combined dimethylsulfoxide-ammonium fluoride treatment; discussion. Clays and Clay Minerals 40(6): 740-741

Anonymous, 1982: Community participation in family planning: issues and examples. This Working Paper summarizes some of the experience which has been gained by the IPPF in community participation in family planning programmes. In the second part of the paper seven projects are described as examples of projects which have genera...

Evans, M., 1952: Precipitation and sorghum yields. Precipitation records from 14-17 weather stations in countries on the eastern border of New Mexico were analysed during 1940-51 to determine whether relationships between precipitation and yields of grain and forage sorghums and broomcorn could be...

Eremenko, Ev; Sinel'-Shchikov, Vs; Loskutov, Nf, 1974: Effect of heated water of thermal electric power plants on the oxygen regime of a stream. Water resources: 1 (5) 774-779

Tegner R., 1989: Tactile sensibility in parietal lesions. The response to tactile stimuli at the threshold range was studied with a forced choice technique in two patients with stable parietal lesions. The most unexpected finding was that very weak stimuli were significantly better detected on the side c...

Ristow H J.; Hoffmann R.; Frank W.; Paul D., 1981: Phospho lipids with special emphasis on changes in phosphatidyl inositol that accompany changes in cellular growth state. Waymouth, C , R G Ham And P J Chapple (Ed ) The Growth Requirements Of Vertebrate Cells in Vitro Xiii+542p Cambridge University Press: New York, N Y , Usa; Cambridge, England Illus P313-326