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Preparation of high density lipo proteins labeled exclusively at the cholesteryl ester moiety


, : Preparation of high density lipo proteins labeled exclusively at the cholesteryl ester moiety. Clinica Chimica Acta 123(1-2): 139-144

The in vitro preparation of [human] HDL [high density lipoprotein] radiolabeled at the cholesteryl ester moiety was determined by incubating different serum fractions with [3H]cholesteryl linoleate or cholesteryl [14C]oleate dissolved in dimethylsulfoxide (DMSO) and subsequent reisolation of HDL from the incubation mixture by ultracentrifugation. Using the supernatant obtained by precipitation of apolipoprotein B-containing lipoproteins with phosphotungstate/MgCl2 or using the > 1.075 kg/l fraction of sera, the bulk of the added radioactivity could be recovered in the reisolated HDL fraction, as demonstrated by density gradient ultracentrifugation or by gel chromatography. Incubation experiments using whole sera or the > 1.063 kg/l fraction of sera resulted in the preferential association of the labeled cholesteryl linoleate with apolipoprotein B-containing lipoproteins.

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Accession: 006173210

PMID: 7116635

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Schriewer, H.; Jabs, H.U.; Schultze, J.; Assmann, G., 1982: Preparation of high-density lipoproteins labelled exclusively at the cholesteryl ester moiety. The in vitro preparation of HDL radiolabelled at the cholesteryl ester moiety was determined by incubating different serum fractions with [3H]cholesteryl linoleate or cholesteryl [14C]oleate dissolved in dimethylsulfoxide (DMSO) and subsequent re-...

Weizel A., 1972: Inclusion of 4 carbon 14 cholesterol in the cholesteryl ester fractions of high density lipo proteins low density lipo proteins and very low density lipo proteins in liver cirrhosis. Schlegel, B (Ed ) Verhandlungen Der Deutschen Gesellschaft Fuer Innere Medizin (Proceedings Of The German Association For Internal Medicine ) Wiesbaden, West Germany April 9-13, Xxviii+1666p Illus Verlag Von J F Bergmann: Muenchen, West Germany 1365-1367

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