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Preparation of highly immunogenic protein conjugates by direct coupling to glutaraldehyde treated cells comparison with commonly used preparations


Journal of Immunological Methods 40(2): 209-218
Preparation of highly immunogenic protein conjugates by direct coupling to glutaraldehyde treated cells comparison with commonly used preparations
A simple and rapid method of preparing highly immunogenic protein samples by use of glutaraldehyde for direct coupling to cells is described. The usefulness of the technique is illustrated in 2 model preparations [in rabbits and mice] based on detoxification [antitoxin production] with tetanus toxin coupling to L1210 leukemia cells or to lymphocytes.

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Accession: 006173227

PMID: 6166687



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