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Preparation of highly purified cephalexin amidase from xanthomonas

, : Preparation of highly purified cephalexin amidase from xanthomonas. Antibiotiki (Moscow) 29(3): 175-178

A procedure for highly purifying cephalexin amidase from Xanthomonas was developed. It consists of preparation of a cell-free extract of the culture after cell disintegration, precipitation with (NH4)2 SO4, dissolution, concentration and elimination of ballast proteins, gel filtration on Sephadex G-25, sorption of ballast proteins on DEAE cellulose and chromatography on CM-cellulose. The enzyme yield is 45-55%. The purity level is 80- to 90-fold.

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