+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of highly purified cephalexin amidase from xanthomonas

Antibiotiki (Moscow) 29(3): 175-178
Preparation of highly purified cephalexin amidase from xanthomonas
A procedure for highly purifying cephalexin amidase from Xanthomonas was developed. It consists of preparation of a cell-free extract of the culture after cell disintegration, precipitation with (NH4)2 SO4, dissolution, concentration and elimination of ballast proteins, gel filtration on Sephadex G-25, sorption of ballast proteins on DEAE cellulose and chromatography on CM-cellulose. The enzyme yield is 45-55%. The purity level is 80- to 90-fold.

Accession: 006173233

Related references

Isolation of a highly pure preparation of cephalexin amidase from bacteria of the genus Xanthomonas. Antibiotiki 29(3): 175-178, 1984

Production of a highly purified diphtheria anatoxin preparation and its characteristics. III. Immunological activity of highly purified diphtheria anatoxin. Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii 33: 14-18, 1962

Preparation and the properties of a highly purified preparation of myosin from smooth muscles. Ukrains'kyi Biokhimichnyi Zhurnal 48(5): 597-602, 1976

Preparation of highly purified electrophoretically homogeneous human growth hormone from a commercial STH preparation. Endokrinologie 55(1): 56-65, 1969

Physiological importance of micro villi bound leucine aryl amidase in the final digestion of proteins comparison of the exo peptidic activities of purified leucine aryl amidase purified micro villi and micro villi sediment. Nahrung 24(8): 735-740, 1980

On the technic of Sakel's insulin cure. Comparative research on a highly purified and a non-purified insulin preparation. Der Nervenarzt 31: 559-560, 1960

Preparation of highly purified I-131 hippuran. Vnitrni Lekarstvi 11(12): 1211-1215, 1965

Preparation of highly purified thrombin. Pharmazeutische Zentralhalle für Deutschland 95(10): 392-396, 1956

Preparation of highly purified secretin. Arkiv Kemi 15(1): 69-74, 1960

Preparation of highly purified cephamycin C. Japanese Journal of Antibiotics 35(7): 1699-1700, 1982