EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of highly purified myosin from mantle muscle of squid ommastrephes sloani pacificus


Nippon Suisan Gakkaishi 46(7): 885-892
Preparation of highly purified myosin from mantle muscle of squid ommastrephes sloani pacificus
Myosin was isolated from the squid mantle muscle in a high yield by Ebashi's method which has been used for preparation of chicken gizzard myosin. The squid myosin was homogeneous on ultracentrifugal sedimentation. The sedimentation constant (s20,w) was estimated to be 6.0 S corresponding to the monomeric myosin. The sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns showed that the squid myosin contained protein components corresponding to a heavy chain and 2 light chains with MW of 15,000 and 17,000. The ATPase activity of squid myosin differed from that of rabbit skeletal myosin in specific activity and KCl concentration dependence. The squid myosin was similar to that of rabbit myosin in actin activation of its Mg-ATPase activity. The Ca-ATPase activity of squid myosin was approximately 50 times more sensitive to heat-inactivation, like that of rabbit myosin.


Accession: 006173239



Related references

Myosin linked calcium regulation in squid ommastrephes sloani pacificus mantle muscle light chain components of squid myosin. Journal of Biochemistry (Tokyo) 86(6): 1639-1650, 1979

Proteolytic enzymes of squid ommastrephes sloani pacificus mantle muscle. Comparative Biochemistry & Physiology B 68(3): 389-396, 1981

Two calcium regulation systems in squid (Ommastrephes sloani pacificus) muscle. Preparation of calcium-sensitive myosin and troponin-tropomyosin. Journal of Biochemistry 84(6): 1431-1440, 1978

Acid proteinase activity of squid ommastrephes sloani pacificus mantle muscle some properties and sub cellular distribution. Comparative Biochemistry & Physiology B 70(4): 791-794, 1981

Characterization of atpase ec 3.6.1.3 of squid ommastrephes sloani pacificus acto myosin. Nippon Suisan Gakkaishi 46(12): 1533-1538, 1980

Physicochemical properties of squid ommastrephes sloani pacificus tropo myosin. Nippon Suisan Gakkaishi 46(7): 893-896, 1980

Physicochemical properties of squid ommastrephes sloani pacificus para myosin. Nippon Suisan Gakkaishi 46(2): 197-200, 1980

Separation of cathepsin D-like proteinase and acid thiol proteinase of squid mantle muscle Todarodes (Ommastrephes sloani) pacificus. Comparative biochemistry and physiology B Comparative biochemistry5(3): 409-414, 1983

Studies on the freeze denaturation of squid ommastrephes sloani pacificus acto myosin. Nippon Suisan Gakkaishi 47(11): 1499-1506, 1981

Studies on the complete utilization of squid (Ommastrephes sloani pacificus). V. Nutritive and digestibility of squid meat. Bulletin of the Faculty of Fisheries Hokkaido, 3: 75-80, 1952