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Preparation of highly purified myosin from mantle muscle of squid ommastrephes sloani pacificus


, : Preparation of highly purified myosin from mantle muscle of squid ommastrephes sloani pacificus. Nippon Suisan Gakkaishi 46(7): 885-892

Myosin was isolated from the squid mantle muscle in a high yield by Ebashi's method which has been used for preparation of chicken gizzard myosin. The squid myosin was homogeneous on ultracentrifugal sedimentation. The sedimentation constant (s20,w) was estimated to be 6.0 S corresponding to the monomeric myosin. The sodium dodecyl sulfate-polyacrylamide gel electrophoretic patterns showed that the squid myosin contained protein components corresponding to a heavy chain and 2 light chains with MW of 15,000 and 17,000. The ATPase activity of squid myosin differed from that of rabbit skeletal myosin in specific activity and KCl concentration dependence. The squid myosin was similar to that of rabbit myosin in actin activation of its Mg-ATPase activity. The Ca-ATPase activity of squid myosin was approximately 50 times more sensitive to heat-inactivation, like that of rabbit myosin.

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