+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of human erythrocyte ghosts in isotonic solution hemo globin content and poly peptide composition

, : Preparation of human erythrocyte ghosts in isotonic solution hemo globin content and poly peptide composition. Zeitschrift fuer Naturforschung Section C Journal of Biosciences 32(7-8): 627-631

Human red blood cell ghosts were prepared by dielectric breakdown of the cell membrane and subsequent electrical hemolysis in isotonic and isoionic solutions. Almost complete removal of Hb (to about 0.1%) was achieved by subjecting the erythrocytes suspended in isotonic solution to 3 consecutive electric field pulses of 16 kV/cm in the presence of 10 mM EDTA, followed by dilution with EDTA-free isoionic medium. The cup-shaped ghost cells were electrically homogeneous. The polypeptide composition of the electrically prepared ghost cells, as analyzed by polyacrylamide electrophoresis in 1% SDS [sodium dodecyl sulfate], revealed a pattern similar to ghost cells prepared by osmotic hemolysis. The band 6, corresponding to glyceraldehyde-3-phosphate-dehydrogenase protomer, was absent from the electrically prepared ghost cells. Also the band 8, which is eluted at low ionic strength, appeared to be very prominent in the electrical preparations.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006173263

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Rowe J.B., 1986: Metabolism of lactic acid isomers in the rumen of silage fed sheep. 1. Four mature sheep were offered perennial ryegrass (Lolium perenne, cv. S23) silage (885 g dry matter/d) at hourly intervals. The silage was well fermented with a pH of 4.0, a lactic acid content of 139 g/kg dry matter and an organic matter dige...

Kalbag S.S.; Palekar A.G., 1990: Postnatal development of the glycine cleavage system in rat liver. The development of the glycine cleavage system was studied in liver mitochondrial extracts from neonatal and adult rats. The enzyme activity in 2-day-old pups was 29.3% of that measured in the adult and was found to increase in an age-dependent ma...

Nilsson L A., 1984: Incidence and prevalence of dermatitis herpetiformis in western sweden. The annual incidence of IgA-positive dermatitis herpetiformis (DH) during 1976-1981 in Gothenburg, Sweden, a city with .apprx. 430,000 inhabitants, was estimated prospectively. The mean annual incidence was 1.1/105 inhabitants. The prevalence of D...

Kisteer, D., 1967: A revision of the Myrmecophilous tribe Deremini Part 2 Additions and corrections to the Dorylopora complex Coleoptera Staphylinidae. Pan-Pacific Entomologist, 43: 274-284

Nisijima K.; Ishiguro T., 1990: Neuroleptic malignant syndrome a study of csf monoamine metabolism. In 8 cases of typical neuroleptic malignant syndrome (NMS), homovanillic acid (HVA), 5-hydroxyindole acetic acid (5-HIAA), noradrenaline (NA),and 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) levels in the cerebrospinal fluid (CSF) were assayed d...

Sobota J.T.; Hirasawa Y., 1991: Recombinant human erythropoietin epoetin beta in patients with end stage renal disease united states and japan. Erslev, A J , Et Al Johns Hopkins Series in Contemporary Medicine And Public Health: Erythropoietin: Molecular, Cellular, And Clinical Biology Xv+478p Johns Hopkins University Press: Baltimore, Maryland, Usa; London, England, Uk Illus 269-290

Mann D.; Leathem J.H., 1968: Reproductive potential following gold thio glucose administration to neo natal mice. Proceedings of the Pennsylvania Academy of Science 42: 230-231

Li Ke, 2005: The history of forest legislation in China and amendment of Forest Law. The history of forest legislation shows that giving more importance to ecology and commonwealth is a general tendency of Forest Law. One way of amending Forest Law is to improve the forest ecological system and realize the combination of forestry...

Gosswald, K., 1973: Ant research areas in Nordrhein-Westfalen. I. Kleve and Bielefeld (Minden). In the Kleve region, large areas of Scots Pine were artificially colonized with ants of strains of Formica polyctena from 1954 onwards. In Bielefeld, the forests concerned were mostly pure Spruce, the majority mature; colonization with F. polycten...

Eckermann P., 1981: The carotid bodies of spontaneously hypertensive rats a functional and morphologic study. The parameters of the acid-base-state of the arterial blood were measured in spontaneously hypertensive rats of the Okamoto-strain (SHR) and in normotensive Wistar rats (NWR) of a random-bred strain. The animals were anesthetized with chloralose-u...