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Preparation of human salivary alpha amylase ec 3.2.1.1 specific monoclonal antibody


, : Preparation of human salivary alpha amylase ec 3.2.1.1 specific monoclonal antibody. Journal of Biochemistry (Tokyo) 97(5): 1357-1362

A mouse hybridoma cell line which produced an anti-human salivary .alpha.-amylase monoclonal antibody was obtained by fusion between mouse spleen cells immunized with human salivary .alpha.-amylase and mouse myeloma cells, followed by screening the hydridoma cells by enzyme-linked immunosorbent assay. The hydridoma cell line (27-4-1) secreted IgG. The monoclonal antibody produced by the hybridoma showed no inhibitory effect on the activity of human salivary .alpha.-amylase. The specificity and reactivity of this monoclonal antibody were examined by determining the activities of human salivary and pancreatic .alpha.-amylases bound to the monoclonal antibody immobilized on polystyrene balls or by enzyme immunoassay with the monoclonal antibody conjugated with .beta.-D-galactosidase. The monoclonal antibody produced by the hydridoma cell line was evidently specific for salivary .alpha.-amylase and absolutely unreactive to pancreatic .alpha.-amylase.

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