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Preparation of hybrid proteins from subunits of sesame 13s globulins and soybean 11s globulins and their molecular weights


Agricultural & Biological Chemistry 45(4): 809-816
Preparation of hybrid proteins from subunits of sesame 13s globulins and soybean 11s globulins and their molecular weights
Combination of the sesame acidic subunit (SesA) and the soybean cv. Tsuranoko basic subunit (SoyB) gave a 1(AB)-type intermediary subunit bound with disulfide linkages and a new 6(AB)-type hybrid protein which had a MW of 265,000 and a sedimentation constant of 9.6S in 20% glycerol. The combination of soybean acidic subunit (SoyA1 or SoyA2) and sesame basic subnit (SesB) gave only 1(AB)-type intermediary subunits. SoyA1-SoyB formed a 6(AB)-type protein whose MW was .apprx. 310,000.


Accession: 006173277



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