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Preparation of immobilized gluco amylase by ion exchange sorption on deae cellulose


, : Preparation of immobilized gluco amylase by ion exchange sorption on deae cellulose. Prikladnaya Biokhimiya i Mikrobiologiya 10(6): 856-860

The method of ion exchange sorption was used for glucoamylase [Endomycopsis sp. 20-9] immobilization. The effect of various factors on the sorption process was examined. An increase in the ionic strength of the solutions reduced the activity of the immobilized enzyme. This activity was shown to depend also on pH: maximum activity occurring at pH 6.5; and activity declined as the pH was shifted either to the acid or alkaline zone. In order to obtain active immobilized glucoamylase, the optimum temperature is 5.degree. C and the optimum time of the carrier-enzymic solution contact is 15 min.

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