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Preparation of immobilized gluco amylase by ion exchange sorption on deae cellulose

, : Preparation of immobilized gluco amylase by ion exchange sorption on deae cellulose. Prikladnaya Biokhimiya i Mikrobiologiya 10(6): 856-860

The method of ion exchange sorption was used for glucoamylase [Endomycopsis sp. 20-9] immobilization. The effect of various factors on the sorption process was examined. An increase in the ionic strength of the solutions reduced the activity of the immobilized enzyme. This activity was shown to depend also on pH: maximum activity occurring at pH 6.5; and activity declined as the pH was shifted either to the acid or alkaline zone. In order to obtain active immobilized glucoamylase, the optimum temperature is C and the optimum time of the carrier-enzymic solution contact is 15 min.

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Accession: 006173292

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Related references

Oreshkin, E.N.; Nakhapetian, L.A.; Vaĭner, L.M., 1974: Preparation of immobilized glucoamylase by means of ion-exchange sorption on DEAE-cellulose. Prikladnaia Biokhimiia i Mikrobiologiia 10(6): 856-860

Anonymous, 1973: Studies on immobilized enzyme part 1 the preparation of immobilized gluco amylase ec 3213 by adsorption on deae sephadex

Monosov, E.Z.; Oreshkin, E.N.; Nakhapetyan, L.A., 1976: Study on localization of gluco amylase in ion exchange immobilization on deae cellulose. Distribution of glucoamylase immobilized on DEAE cellulose was studied by scanning electron and fluorescent microscopy. Protein complexes on carrier were determined by contrasting them with pairs of OsO4. Significance of microdislocations on carri...

Anonymous, 1973: Studies on immobilized enzyme part 2 preparation of p amino benzene sulfonylethyl cellulose gluco amylase ec 3213 complex

Jach, M.; Sugier, H., 1983: Adsorption of gluco amylase ec on deae cellulose. The adsorption of glucoamylase on DEAE-cellulose and the properties of the immobilized enzyme in respect to its use in hydrolysis of starch are described.

Sharma, K.K.; Pattabiraman, T.N., 1982: Purification of porcine pancreatic amylase by affinity chromatography with amylase inhibitor of Dioscorea alata immobilized on DEAE-cellulose. Indian Journal of Biochemistry & Biophysics 19(1): 54-56

Anonymous, 1982: Purification of porcine pancreatic amylase ec 3211 by affinity chromatography with amylase inhibitor of dioscorea alata immobilized on deae cellulose

Bohnenkamp C.G.; Reilly P.J., 1980: Use of immobilized gluco amylase beta amylase and gluco amylase fungal amylase mixtures to produce high maltose syrups. Biotechnology & Bioengineering 22(8): 1753-1758

Roth, P.; Feist, U.; Flemming, C.; Gabert, A.; Taeufel, A., 1977: Synthesis and properties of immobilized enzymes part 7 coupling of gluco amylase ec to di aldehyde cellulose carboxymethyl cellulose hydrazide and carboxymethyl cellulose azide. Glucoamylase (.alpha.-1,4-glucan glucohydrolase, EC was covalently linked to dialdehyde cellulose resulting in an immobilized enzyme containing 0.98% protein and an activity of 4.5 mg of the native enzyme/g of matrix, i.e., 46% relative a...

Anonymous, 1978: Gluco amylase ec 3213 immobilized on aminoethyl cellulose