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Preparation of immobilized proteolytic enzymes on co polymers of ethylene with maleic anhydride


, : Preparation of immobilized proteolytic enzymes on co polymers of ethylene with maleic anhydride. Prikladnaya Biokhimiya i Mikrobiologiya 13(1): 88-90

The covalent immobilization of trypsin, chymotrypsin and papain on copolymers of ethylene with maleic anhydride with different molecular weight and divinyl benzene content was studied. The optimum concentration of enzyme that maintained 50% activity after immobilization was determined. The shift of pH and temperature optima of dissolved and immobilized enzymes was examined. The esterase activity of immobilized trypsin and chymotrypsin with respect to dissolved enzymes was measured.

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