EurekaMag.com logo
+ Translate

Preparation of immobilized soybean beta amylase on porous cellulose beads and continuous maltose production/


, : Preparation of immobilized soybean beta amylase on porous cellulose beads and continuous maltose production/. Biotechnology and Bioengineering 20(3): 383-402

Immobilized soybean .beta.-amylase was prepared by using porous cellulose beads. The expressed activity of the .beta.-amylase-cellulose beads conjugated below 35 mesh was 59-69% of the initial activity and the protein content was 10-13%. General properties of the conjugate were almost identical with those of the native enzyme except for the Km value. The Km value of the conjugate was 40 mM and the Km value of the native enzyme was 0.6 mM. This large difference was probably caused by pore structure, i.e., a pore diffusion problem. The film diffusion problem occurred at the flow rate below a linear velocity of 3 cm/min. Maximum maltose contents of the hydrolyzates prepared by the conjugate and the native enzyme were 69% and 71%, respectively. After a continuous column operation at 50.degree. C for 17 days, the activity of the column was 60% of the initial activity. The half-life of the column at 40.degree. C was 40 days.

(PDF 0-2 workdays service)

Accession: 006173299

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Noda, T.F.ruta, S.S.da, I., 2001: Sweet potato beta-amylase immobilized on chitosan beads and its application in the semi-continuous production of maltose. The present study is concerned with the immobilization of beta-amylase from sweet potato tuberous roots on chitosan beads for the purpose of maltose production. Immobilized sweet potato beta-amylase on Chitopearl BCW 3505 beads exhibited an activi...

Anonymous, 1974: Preparation of an immobilized 2 enzyme system beta amylase pullulanase to an acrylic co polymer for the conversion of starch to maltose part 1 preparation and stability of immobilized beta amylase ec 3212

Mårtensson, K., 1974: Preparation of an immobilized two-enzyme system, Beta-amylase--pullulanase, to an acrylic copolymer for the conversion of starch to maltose. III. Process kinetic studies on continuous reactors. Biotechnology and Bioengineering 16(12): 1567-1587

Martensson K., 1974: Preparation of an immobilized 2 enzyme system beta amylase pullulanase to an acrylic co polymer for the conversion of starch to maltose part 3 process kinetic studies on continuous reactors. Biotechnology & Bioengineering 16(12): 1567-1587

Mårtensson, K., 1974: Preparation of an immobilized two-enzyme system, beta-amylase-pullulanase, to an acrylic copolymer for the conversion of starch to copolymer for the conversion of starch to maltose. I. Preparation and stability of immobilized beta-amylase. Biotechnology and Bioengineering 16(5): 567-577

Hausser, A.G.; Goldberg, B.S.; Mertens, J.L., 1983: An immobilized 2 enzyme system fungal alpha amylase ec 3.2.1.1 gluco amylase ec 3.2.1.3 and its use in the continuous production of high conversion maltose containing corn syrups. Fungal .alpha.-amylase and glucoamylase were chemically attached to separate reactor modules made from Microporous Plastic Sheet (MPS). Immobilization of enzymes and subsequent chemical reactions were accomplished by pumping reactants through the...

Deleyn F.; Stouffs R., 1990: Immobilized beta amylase in the production of maltose syrups. In order to have a full continuous starch hydrolysates production process, the use of continuous conversions catalysed by immobilised enzymes is necessary. With respect to this, immobilisation of .beta.-amylase on anion exchange resins and continu...

Szajani, B.B.zas, Z.D.llmann, K.G.mesi, I.K.isch, J.T.th, M., 1996: Continuous production of ethanol using yeast cells immobilized in preformed cellulose beads. Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. The fermentation capacity of the immobilized yeast cells was found to be practically independent of the hydrogen ion concentration between pH 3.1 and 6.25....

Takagi, M.; Shirokaze, J.I.hi; Oishi, K.; Otsubo, K.; Yamamoto, K.; Yoshida, N.; Fujimatsu, I., 1994: Production of S-dextro-ibuprofen with high optical purity from a nitrile compound by cells immobilized on cellulose porous beads. The production of S-(+)-ibuprofen (S-IP) from a nitrile compound by immobilized cells was investigated. Cells immobilized within both kappa-carrageenan and Ca-alginate showed a lower reaction conversion and gave a product of lower optical purity t...

Hausser, A.G.; Goldberg, B.S.; Mertens, J.L., 1983: An immobilized two-enzyme system (fungal alpha-amylase/glucoamylase) and its use in the continuous production of high conversion maltose-containing corn syrups. Fungal alpha-amylase (E.C. 3.2.1.1) and glucoamylase (E.C. 3.2.1.3) were chemically attached to separate reactor modules made from Microporous Plastic Sheet (MPS). Immobilization of enzymes and subsequent chemical reactions were accomplished by pu...