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Preparation of iodine 125 27 monoiodotyrosine 5 leucine beta h endorphin and its use for crosslinking of opioid binding sites in human striatum and ng 108 15 neuroblastoma glioma cells

, : Preparation of iodine 125 27 monoiodotyrosine 5 leucine beta h endorphin and its use for crosslinking of opioid binding sites in human striatum and ng 108 15 neuroblastoma glioma cells. Proceedings of the National Academy of Sciences of the United States of America 83(13): 4622-4625

A radioligand suitable for crosslinking studies to opoid receptors has been obtained by radioiodination and purification of the monoiodotyrosine-27 derivative of the synthetic human .beta.-endorphin (.beta.h-endorphin) analogue [5-leucine].beta.h-endorphin. The derivative, [27-[125I]monoiodotyrosine, 5-leucine].beta.h-endorphin, was crosslinked to human striatal (caudate and putamen) and NG105-15 neuroblastoma-glioma cell membranes by using disuccinimidyl suberate. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis under reducing conditions revealed four specifically labeled bands at 68, 40, 30, and 25 kDa for both human caudate and putamen, whereas NG108-15 cell membranes gave specifically labeled bands at 92, 56, 38, and 23 kDa.

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