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Preparation of iodine 125 labeled 3 tyrosine neurotensin and iodine 125 labeled 11 tyrosine neurotensin for radio immunoassay

, : Preparation of iodine 125 labeled 3 tyrosine neurotensin and iodine 125 labeled 11 tyrosine neurotensin for radio immunoassay. Scandinavian Journal of Clinical & Laboratory Investigation 43(6): 483-492

The chloramine-T method for radioiodination of neurotensin for radioimmunoassay [in humans] was studied. As conventional procedures produced heterogeneous preparations, labeling was performed with a low amount of chloramine-T (1.8 nmol) in the presence of excess of peptide (6 nmol). Purification and complete separation of labeled from unlabeled peptide was obtained by ion-exchange chromatography on SP Sephadex C-25. Four labeled components were identified by isoelectric focusing, enzymatic cleavage and studies of immunoreactivity. The 2 components representing monoiodinated preparations labeled at Tyr 3 or Tyr 11 could be isolated. Depending on the binding site of the particular antiserum the appropriate tracer could be selected for use in the radioimmunoassay. The specific radioactivities were high (2303 (2137-2407) .mu.Ci/nmol and 1927 (1608-2307) .mu.Ci/nmol (median and range)) and the stability of the label and the reproducibility of the procedure was good.

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