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Preparation of iodine 125 labeled gastric inhibitory poly peptide using water insoluble 1 3 4 6 tetra chloro 3 alpha 6 alpha diphenyl glycoluril iodogen as the oxidizing agent


Scandinavian Journal of Gastroenterology 17(3): 379-382
Preparation of iodine 125 labeled gastric inhibitory poly peptide using water insoluble 1 3 4 6 tetra chloro 3 alpha 6 alpha diphenyl glycoluril iodogen as the oxidizing agent
The preparation and evaluation of 125I-labeled gastric inhibitory polypeptide (GIP) by means of a modified Iodo-gen method are described. The portal vein fasting plasma GIP concentration was determined in 6 groups of rats with 10 rats in each group. In 3 of the groups, 3 separately prepared Iodo-gen tracers were used in the radioimmunoassay, and in the 3 other groups, 3 separately prepared chloramine-T tracers were used. The mean fasting portal vein plasma GIP concentrations in the 3 groups were 45.0 .+-. 5.0, 41.9 .+-. 3.9, and 39.6 .+-. 2.7 pmol/l, respectively, with the Iodo-gen tracers and 80.3 .+-. 4.6, 78.4 .+-. 5.4, and 85.7 .+-. 3.7 pmol/l with the chloramine-T tracers. The values obtained in each of the groups with the Iodo-gen tracers were significantly lower than those obtained in each of the groups with the chloramine-T tracers. This may be due to a better preserved immunoreactivity of the Iodo-gen tracer than of the chloramine-T tracer. The explanation of this may be the water insolubility of Iodo-gen, which may prevent oxidation of easily oxidized residues of the peptide during the radioiodination procedure.


Accession: 006173347



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