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Preparation of iodine 125 labeled human fibroblast interferon


, : Preparation of iodine 125 labeled human fibroblast interferon. Journal of General Virology 40(3): 681-684

Human fibroblast interferon was labeled with 125I using the Bolton-Hunter reagent. Purification of the interferon after iodination gave a product which had stable biological activity for 1 mo. and had a specific radioactivity of 2-4 .mu.Ci/.mu.g.

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Fuse A.; Ashino Fuse H.; Kuwata T., 1984: Binding of iodine 125 labeled human interferon to cell lines with low sensitivity to interferon. Iodinated human interferon .alpha.A (HuIFN-.alpha.A) was found to bind specifically to IFN-sensitive RSa [human embryonal carcinoma] cells: the dissociation constant was 8.7 .times. 10-10 M and the estimated number of receptor sites per cell was 1...

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