+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of iodine 125 labeled synthetic porcine secretin for radio immunoassay

, : Preparation of iodine 125 labeled synthetic porcine secretin for radio immunoassay. Scandinavian Journal of Clinical and Laboratory Investigation 36(7): 661-668

Synthetic porcine secretin was labeled by the conjugation-labeling method of Bolton and Hunter, the lactoperoxidase method, the gaseous diffusion method and the chloramine-T method. The chloramine-T technique was adapted as the routine method. Ten .mu.g (3.27 nmol) peptide was reacted with 5 mCi of Na125I at a concentration of chloramine-T of 1.3 mmol/l. Synthetic secretin was suitable for labeling for at least 8 mo. when stored as dry matter in N-filled glass ampoules. Purification and separation of labeled from unlabeled hormone was carried out by gel-permeation chromatography on Sephadex G-50 superfine. The labeled preparation had a specific radioactivity of 405 .+-. 33 .mu.Ci/nmol (mean .+-. SEM [standard error of the mean], n = 9) and was usable for 6 wk. 6-Tyrosyl-secretin took up more I compared to porcine synthetic secretin but had lower immunoreactivity with all antisera tested.

Accession: 006173358

Submit PDF Full Text: Here

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Stanczyk F.Z.; Goebelsmann U., 1981: Use of iodine 125 labeled iodo histamine labeled steroid derivatives as radio ligands for radio immunoassay of natural and synthetic steroids. [125I]-Iodohistamine-labeled steroid derivatives were prepared and utilized as radioligands in radioimmunoassays of progesterone, testosterone, estradiol, estriol, estriol-16.alpha.-glucuronide, levonorgestrel, norethindrone and medroxyprogesteron...

Baur, S.; Bacon, V.C.; Rosenquist, G.L., 1978: Preparation and use of iodine 125 labeled synthetic human 15 leucine gastrin 1 17 i for radio immunoassay of conventional human gastrins/. A method of radiodination and use of synthetic human (15-Leu)gastrin in radioimmunoassay for synthetic or naturally occurring human gastrin is described. Specific activity of the [125I]monoiodo(15-Leu)gastrin was 45% higher than that obtained with...

Boden G.; Chey W.Y., 1973: Preparation and specificity of anti serum to synthetic secretin and its use in a radio immunoassay. Endocrinology 92(6): 1617-1624

Pitt, C.G.; Seltzman, H.H.; Setzer, S.R.; Williams, D.L., 1980: Preparation of iodine 125 labeled 5' iodo delta 8 tetra hydro cannabinol a radio ligand for the radio immunoassay of cannabinoids. Nucleophilic exchange of 5'-iodo-.DELTA.8-THC (5'-iodo-.DELTA.8-tetrahydrocannabinol) and sodium iodide-125 afforded 5'-iodo-125I-.DELTA.8-THC with a maximum specific activity of 42 Ci/mmol. The specific activity could be increased...

Hanson V.W.; Buonarati M.H.; Baselt R.C.; Wade N.A.; Yep C.; Biasotti A.A.; Reeve V.C.; Wong A.S.; Orbanowsky M.W., 1983: Comparison of tritium radio immunoassay labeled and iodine 125 labeled radio immunoassay and gas chromatography mass spectrometry for the determination of delta 9 tetra hydro cannabinol and cannabinoids in blood and serum. Blood and serum specimens from 100 subjects in a driving study were tested for the presence of .DELTA.9-tetrahydrocannabinol (THC) [the psychoactive component of marijuana] by 3H- and 125I-labeled RIA [radioimmunoassay] and GC/MS [gas chromatograp...

Warren R.J.; Fotherby K., 1975: Iodine labeled ligands in the radio immunoassay of synthetic progestins. Journal of Steroid Biochemistry 6(7): 1151-1156

de Muckadell, O.B.; Fahrenkrug, J., 1976: Preparation of 125I-labeled synthetic porcine secretin for radioimmunoassay. Synthetic porcine secretion was labelled by the conjugation-labelling method of Bolton & Hunter, the lactoperoxidase method, the gaseous diffusion method, and the chloramine-T method. The chloramine-T technique was adapted as routine method. T...

Cardoso M.T.; Pradelles P., 1982: Preparation of iodine 125 labeled n 1 ethyl 2 pyrrolidylmethyl 2 methoxy 4 iodo 5 ethylsulfonyl benzamide a radio ligand for the radio immunoassay of sulpiride related compounds. The preparation of 125I labeling with a higher specific radioactivity of N(1-ethyl-2-pyrrolidyl-methyl)2-methoxy-5-ethyl sulfonyl benzamide, a potent biological analog for sulpiride, a psychoactive drug, is described. The incorporation of I in the...

Wal J M.; Kann G., 1975: Preparation of a iodine 125 labeled derivative of penicillin to be used for radio immunoassay. Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences Serie D Sciences Naturelles 280(20): 2405-2408

Massaglia A.; Barbieri U.; Siri Upathum C.; Vitali R., 1973: Preparation of iodine 125 labeled steroid derivatives for radio immunoassay. International Journal of Applied Radiation & Isotopes 24(8): 455-462