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Preparation of iodine 125 poly peptide hormones for radio immunoassay using glucose oxidase with lacto peroxidase


Life Sciences 21(7): 959-966
Preparation of iodine 125 poly peptide hormones for radio immunoassay using glucose oxidase with lacto peroxidase
The hydrogen peroxide-generating property of glucose oxidase was used in combination with the catalytic property of lactoperoxidase to radioiodinate polypeptide hormones [human prolactin, thyrotropin, luteinizing hormone, follicle stimulating hormone and growth hormone] with 125I. This rapid, gentle, enzymatic method reproducibly results in higher yields of iodinated hormones for use in radioimmunoassays.


Accession: 006173360



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