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Preparation of labeled phospho lipids acylation of 2 lyso phosphatidyl choline with 9 10 tritium labeled oleic acid/

Journal of Labelled Compounds & Radiopharmaceuticals 21(6): 497-506

Preparation of labeled phospho lipids acylation of 2 lyso phosphatidyl choline with 9 10 tritium labeled oleic acid/

1-Acyl-2-[9,10-3H]oleoyl-sn-glycero-3-phosphorylcholine was prepared by acylating 1-acyl-sn-glycero-3-phosphorylcholine with labeled oleic acid, in the presence of dicyclohexylcarbodiimide (DCC), as condensing agent. A simple column chromatographic procedure allowed both purification of the labeled phospholipid and recovery of the unreacted oleic acid, at the end of the reaction. In standard conditions, yields of 35-40% were obtained. The major feature of this procedure is that it is a one pot reaction not requiring the isolation of an activated acid derivative prior to esterification. This method is also suitable for preparing labeled phospholipids other than phosphatidylcholine, provided that free or unmasked amino or hydroxyl groups are absent in the molecule.

Accession: 006173389

Related references

Gosselin L., 1976: Formation of molecular species of phosphatidyl choline and phosphatidyl ethanolamine by acylation of endogenous lyso phospho lipids in rat liver extracts. Paoletti, Rodolfo, Giuseppe Porcellati And Giovanni Jacini (Ed ) Lipids, Vol 1 Biochemistry Symposia Milan, Italy Sept 1974 Xvi+282p Illus Raven Press: New York, N Y , U S A Isbn 0-89004-028-1 59-66

Okuyama H.; Inoue M., 1982: Preparation and analysis of radio labeled phosphatidyl choline and phosphatidyl ethanolamine containing tritium and carbon 14 labeled poly unsaturated fatty acids. Lands, W E M And W L Smith (Ed ) Methods in Enzymology, Vol 86 Prostaglandins And Arachidonate Metabolites Xxv+705p Academic Press, Inc : New York, N Y , Usa: London, England Illus P370-376

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