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Preparation of lipid vesicles containing high levels of entrapped radioactive cations


Analytical Biochemistry 94(2): 302-307
Preparation of lipid vesicles containing high levels of entrapped radioactive cations
The ionophore A23187 was incorporated into the lipid bilayer of unilamellar vesicles and used to carry externally added In3+ to a chelator, nitrilotriacetic acid, which was previously entrapped in the vesicles. The extent of vesicle loading was assayed by Sephadex chromatography and .gamma.-ray perturbed angular correlation spectroscopy. Incubation conditions were developed which allow rapid loading of more than 90% of added 111In3+ with specific activities of 200-300 .mu.Ci/mg lipid.

Accession: 006173416

PMID: 380400

DOI: 10.1016/0003-2697(79)90364-6

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