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Preparation of liposomes from stratum corneum lipids


, : Preparation of liposomes from stratum corneum lipids. Journal of Investigative Dermatology 87(5): 582-584

Mammalian stratum corneum contains multiple intercellular lipid bilayers that constitute the epidermal water barrier. Unlike all other biologic membranes, the epidermal lamellae do not contain phospholipids, as a result of which the ability of the stratum corneum lipid mixture to form bilayers has been questioned. In the present study, a lipid mixture containing only epidermal ceramides (40%), cholesterol (25%), palmitic acid (25%), and cholesteryl sulfate (10%), approximating the composition of stratum corneum lipids, formed stable, unilamellar liposomes when sonicated at 80.degree. C in buffer containing 100 mM NaCl, 5 mM Tris, and 1 mM EDTA at pH 7.5. The size and form of the liposomes were studied by both freeze fracture and negative staining electron microscopy. Lipid mixtures from which either the palmitic acid or the cholesteryl sulfate were omitted were still capable of forming similar liposomes, but a mixture of ceramides and cholesterol, or ceramides alone, were incapable of forming liposomes. The results indicate that lipid mixtures similar to those found in stratum corneum are capable of forming bilayers at physiologic pH.

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Accession: 006173421

PMID: 3772151

DOI: 10.1111/1523-1747.ep12455832

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D.S.asi, V.; D'Antonio, A.; Caleo, A.; Valvano, L., 2014: Metastatic renal cell carcinoma to the thyroid gland 24 years after the primary tumour. Among the metastases to thyroid gland, metastases from renal cell carcinoma (RCC) are not rare and their frequent macroscopic looks are similar to primary thyroid tumours. We report an unusual case of thyroid metastases from renal carcinoma in a 7...

Wagner, H.; Budweg, W.; Horhammer, L.; Vermes, B.; Farkas, L., 1971: Transacylation reactions in the flavonoid series. VI. Synthesis of luteolin-7-beta-rutinoside (scolymoside) and 3'.4'-di-O-methyl-luteolin-7-beta-neohesperidoside and -7-beta rutinoside. Chemische Berichte 104(7): 2118-2123

Boulton G. S., 1979: A model of Weichselian glacier variation in the North Atlantic region. Boreas: Pages 373-395. 1979.

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SANSONE.G.; ZUNIN.C., 1953: Considerations on 12 cases of renal tumors observed in the last two years at the Clinica Pediatrica di Genova. Minerva Pediatrica 5(14): 594-604

Quinn, D.; Tager, A.; Joseph, P.M.; Bonventre, J.V.; Force, T.; Hales, C.A., 1999: Stretch-induced mitogen-activated protein kinase activation and interleukin-8 production in type II alveolar cells. Chest 116(1 Suppl): 89s-90s

Newcomen, N., 2016: More people behind bars nearing end of life. NlmCategory="UNASSIGNED">As prisons and probation ombudsman, I investigate deaths in prison in England and Wales. Demand for my services has never been greater, with a record 304 deaths to investigate in 2015-16, 42% more than 5 years...

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Noaman, M.M.; Ahmad, E., 2004: Development of alfalfa tolerant to salinity stress using organogenesis technique. The possibility of using organogenesis culture to evaluate salt tolerance of cultivated lucerne (cv. Omani) plants was studied and related to the response obtained by callus culture. Two laboratory experiments (exps) were conducted: callus initiat...

Khan, K.; Steart, B.; Boyle, K.; Gross, D.; Hogan, T.; Antecol, D.; Shuaib, A., 1995: The usefulness of transcranial Doppler in detecting cardiac shunts in patients with TIAs and acute stroke. Neurology 45(4 SUPPL 4): A225