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Preparation of liposomes incorporating membrane components from human lymphoid cells


Tissue Antigens 14(5): 385-397
Preparation of liposomes incorporating membrane components from human lymphoid cells
A procedure for the preparation of liposomes incorporating membrane components of human lymphoid cells is described; the detergent used to solubilize the plasma membrane is almost completely eliminated. MHC [major histocompatibility complex] products HLA-A-B-C and DR molecules were incorporated into the liposomes and their antigenic determinants were exposed on the outer surface. A protein-rich liposomal fraction could be separated from protein-free liposomes and aggregates. This preparation may be suitable for testing functions of cell membrane components in biological assay systems.

Accession: 006173422

PMID: 12731570

DOI: 10.1111/j.1399-0039.1979.tb00867.x

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