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Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate


Zeitschrift fuer Pflanzenphysiologie 87(2): 167-172
Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate
An enzymatic method is described for the preparation of viable and stable protoplasts from cell suspension cultures of Lolium endosperm. A new purification method was developed using a conjugate of Sepharose 4B and the mouse myeloma protein J539 (an anti-galactan agglutinin). The particulate contaminants bound to Sepharose-J539 forming large aggregates which were separated from the protoplast suspension by simple filtration.

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Accession: 006173427



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