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Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate






Zeitschrift fuer Pflanzenphysiologie 87(2): 167-172

Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate

An enzymatic method is described for the preparation of viable and stable protoplasts from cell suspension cultures of Lolium endosperm. A new purification method was developed using a conjugate of Sepharose 4B and the mouse myeloma protein J539 (an anti-galactan agglutinin). The particulate contaminants bound to Sepharose-J539 forming large aggregates which were separated from the protoplast suspension by simple filtration.


Accession: 006173427



Related references

Keller, F.; Stone, B.A., 1978: Preparation of Lolium protoplasts and their purification using an anti-galactan-sepharose conjugate. An enzymatic method is described for the preparation of viable and stable protoplasts from liquid suspension cultures of endosperm cells.

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