EurekaMag.com logo
+ Translate

Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate


, : Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate. Zeitschrift fuer Pflanzenphysiologie 87(2): 167-172

An enzymatic method is described for the preparation of viable and stable protoplasts from cell suspension cultures of Lolium endosperm. A new purification method was developed using a conjugate of Sepharose 4B and the mouse myeloma protein J539 (an anti-galactan agglutinin). The particulate contaminants bound to Sepharose-J539 forming large aggregates which were separated from the protoplast suspension by simple filtration.

(PDF 0-2 workdays service)

Accession: 006173427

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

Keller, F.; Stone, B.A., 1978: Preparation of Lolium protoplasts and their purification using an anti-galactan-sepharose conjugate. An enzymatic method is described for the preparation of viable and stable protoplasts from liquid suspension cultures of endosperm cells.

Creemers-Molenaar, T.L.effen, J.; Zaal, M., 1988: Isolation, culture and regeneration of Lolium perenne and Lolium multiflorum protoplasts. Current plant science and biotechnology in agriculture: ) 53-54

Wang, G.R.; Binding, H.; Posselt, U.K., 1997: Fertile transgenic plants from direct gene transfer to protoplasts of Lolium perenne L. and Lolium multiflorum Lam. A method of direct gene transfer was adapted and applied to protoplasts of the monocots Lolium perenne and Lolium multiflorum. A total of 20 clones, which produced green transgenic plants expressing the transgenes GUS gene (uidA) and neomycin phos...

Anonymous, 1988: Plant regeneration from cell suspension protoplasts of festuca arundinacea schreb lolium perenne l and lolium multiflorum lam

Klinga, K., 1986: Aneuploidy in induced autotetraploid populations of festuca pratensis lolium multiflorum and lolium perenne ii. the frequency of aneuploids and performance of spaced plants in lolium multiflorum and lolium perenne. Frequencies of aneuploids were determined in three induced autotetraploid populations of Lolium, two of L. multiflorum, and one of L. perenne. Vegetative and generative properties of euploids, hypoploids, and hyperploids grown as spaced plants wer...

Carnide V.; Ribeiro M.A.M.D.V.; Pinto H.P.G., 1986: Three years data 1982 1984 collected on spaced plants of 41 cultivars of lolium perenne lolium multiflorum and lolium perenne x lolium multiflorum under the edaphic and climatic conditions of vila real northeast portugal. Revista de Ciencias Agrarias 9(2): 83-96

Heszky, L., 1972: Induction of autotetraploids of Lolium perenne L., Lolium multiflorum Lam., Lolium multiflorum Lam. var. westerwoldicum (Mansh.) Wittm. and Festuca pratensis Huds. by various methods of colchicine treatment. Agrobotanika ub 1972), 13 53-67

Heszky, L., 1971: Embryo sizes in Lolium perenne L., Lolium multiflorum Lam. and Lolium multiflorum Lam. var. westerwoldicum (Mansh.) Wittm. at the diploid and the tetraploid level. Agrobotanika ub 1971), 12 61-70

Anderson, R.L.; Clarke, A.E.; Jermyn, M.A.; Knox, R.B.; Stone, B.A., 1977: A carbohydrate binding arabino galactan protein from liquid suspension cultures of endosperm from lolium multiflorum. Water-soluble arabinogalactan-proteins were isolated from tissue-cultured rye grass endosperm cells and their culture medium by precipitation with the .beta.-glucosyl Yariv artificial antigen. The intracellular and extracellular polymers are simil...

Pollard, P.C.; Fincher, G.B., 1981: Biosynthesis of arabino galactan protein in lolium multiflorum rye grass endosperm cells 1. hydroxylation of peptidyl proline. Suspension-cultured endosperm cells from L. multiflorum secrete into the medium an arabinogalactan-protein in which the protein moiety is rich in hydroxyproline. When cells are grown in the presence of proline labeled with 14C or 3H, the imino aci...