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Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate

, : Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate. Zeitschrift fuer Pflanzenphysiologie 87(2): 167-172

An enzymatic method is described for the preparation of viable and stable protoplasts from cell suspension cultures of Lolium endosperm. A new purification method was developed using a conjugate of Sepharose 4B and the mouse myeloma protein J539 (an anti-galactan agglutinin). The particulate contaminants bound to Sepharose-J539 forming large aggregates which were separated from the protoplast suspension by simple filtration.

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