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Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate


, : Preparation of lolium multiflorum protoplasts and their purification using an anti galactan sepharose conjugate. Zeitschrift fuer Pflanzenphysiologie 87(2): 167-172

An enzymatic method is described for the preparation of viable and stable protoplasts from cell suspension cultures of Lolium endosperm. A new purification method was developed using a conjugate of Sepharose 4B and the mouse myeloma protein J539 (an anti-galactan agglutinin). The particulate contaminants bound to Sepharose-J539 forming large aggregates which were separated from the protoplast suspension by simple filtration.

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Johnson, J.A.; Lott, H.A., 1978: Standardization of the Coomassie Blue method for cerebrospinal fluid proteins. Coomassie Brilliant Blue G-250 can be used to quantitatively determine proteins in cerebrospinal fluid. When the dye combines with protein, the absorption maximum of the dye shifts. The dye-protein color forms almost instantaneously and is stable...

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