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Preparation of low percent poly acrylamide gels for radio isotopic analysis of nucleic acid preparation


, : Preparation of low percent poly acrylamide gels for radio isotopic analysis of nucleic acid preparation. Mikrobiologicheskii Zhurnal (Kiev) 42(6): 783-785

Preliminary drying of low-percent polyacrylamide gels on a polyvinyl chloride plastic provides a wax-like gel which can be easily cut into pieces and can dissolve in a 30% H2O2 water solution with complete extraction of radioactive substances.

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Related references

Grama, D.P., 1980: Preparation of low-percent polyacrylamide gels for the radioisotopic analysis of nucleic acids. Mikrobiologicheskii Zhurnal 42(6): 783-785

Kahn S.N.; Thompson E.J., 1978: Effect of sample preparation on protein patterns in poly acrylamide gels. Clinical Chemistry 24(6): 1014

Jones G.D.; Wilson M.T.; Darley Usmar V.M., 1981: A method for the preparation of low ph dodecyl sulfate poly acrylamide gradient gels. A low-pH lithium dodecyl sulfate/polyacrylamide-gradient slab-gel system, suitable for electrophoresis, is described, and the migration properties of standard proteins are compared on this and conventional high-pH gels. Cytochrome oxidase may be p...

Lee R.T.; Cascio S.; Lee Y.C., 1979: A simple method for the preparation of poly acrylamide gels containing thio glycoside ligands. A simple method was developed for the preparation of acrylamide derivatives containing thioglycosides. The synthetic scheme involves the preparation of an O-acetylated 1-thiosugar via a pseudothiourea derivative, the controlled addition of the thi...

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Ghorbaniazar, P.; Sepehrianazar, A.; Eskandani, M.; Nabi-Meibodi, M.; Kouhsoltani, M.; Hamishehkar, H., 2015: Preparation of Poly Acrylic Acid-Poly Acrylamide Composite Nanogels by Radiation Technique. Nanogel, a nanoparticle prepared from a cross-linked hydrophilic polymer network, has many biomedical applications. A radiation technique has recently been introduced as one of the appropriate methods for the preparation of polymeric nanogels due...

Yu, R.; Zheng, S., 2016: Poly(acrylic acid)-grafted poly(N-isopropyl acrylamide) networks: preparation, characterization and hydrogel behavior. Poly(acrylic acid)-grafted poly(N-isopropylacrylamide) co-polymer networks (PNIPAAm-g-PAA) were prepared via the reversible addition-fragmentation transfer (RAFT) polymerization of N-isopropyl- acrylamide (NIPAAm) with trithiocarbonate-terminated...

Tümtürk, H.; Karaca, N.; Demirel, G.; Sahin, F., 2006: Preparation and application of poly(N,N-dimethylacrylamide-co-acrylamide) and poly(N-isopropylacrylamide-co-acrylamide)/kappa-Carrageenan hydrogels for immobilization of lipase. In the present of this study, two novel polymeric matrixes that are poly(N,N-dimethylacrylamide-co-acrylamide) and poly(N-isopropylacrylamide-co-acrylamide)/kappa-Carrageenan was synthesized and applied for immobilization of lipase. For the immobi...

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