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Preparation of membrane vesicles from isolated myelin studies on functional and structural properties

Biochimica et Biophysica Acta 509(3): 397-409
Preparation of membrane vesicles from isolated myelin studies on functional and structural properties
Myelin membranes purified from bovine brain form membrane vesicles when incubated in hypotonic buffer. Following restoration of isotonicity, a resealing of the membrane occurs as judged by a significant decrease in 22Na+ permeability. ESR measurements using stearic acid spin label I indicate a small decrease in membrane fluidity with increasing ionic strength between 50 and 80 mM NaCl. Iodination of myelin membrane vesicles by lactoperoxidase shows a 4-fold increase in the amount of I incorporation into the myelin basic protein from 0-150 mM NaCl, while the iodination of the proteolipid protein remains essentially unaffected by the change in ionic strength. This dependence of the iodination of the myelin basic protein on the ionic strength can be explained by the electrostatic interactions of this protein with membrane lipids. In view of striking analogies with studies on model membranes correlating protein binding with membrane permeability changes, a similar structure-function relationship for the myelin basic protein is suggested.

Accession: 006173455

PMID: 207322

DOI: 10.1016/0005-2736(78)90234-1

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