EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of membrane vesicles from isolated myelin studies on functional and structural properties


, : Preparation of membrane vesicles from isolated myelin studies on functional and structural properties. Biochimica et Biophysica Acta 509(3): 397-409

Myelin membranes purified from bovine brain form membrane vesicles when incubated in hypotonic buffer. Following restoration of isotonicity, a resealing of the membrane occurs as judged by a significant decrease in 22Na+ permeability. ESR measurements using stearic acid spin label I indicate a small decrease in membrane fluidity with increasing ionic strength between 50 and 80 mM NaCl. Iodination of myelin membrane vesicles by lactoperoxidase shows a 4-fold increase in the amount of I incorporation into the myelin basic protein from 0-150 mM NaCl, while the iodination of the proteolipid protein remains essentially unaffected by the change in ionic strength. This dependence of the iodination of the myelin basic protein on the ionic strength can be explained by the electrostatic interactions of this protein with membrane lipids. In view of striking analogies with studies on model membranes correlating protein binding with membrane permeability changes, a similar structure-function relationship for the myelin basic protein is suggested.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006173455

PMID: 207322

DOI: 10.1016/0005-2736(78)90234-1

PDF Full Text: Preparation of membrane vesicles from isolated myelin studies on functional and structural properties


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

McSweeney, D.J., 1965: A vaginal test for the fertile phase and for ovarian hormone activity. Transactions of the New England Obstetrical and Gynecological Society 19: 127-136

Spongberg, S. A., 1995: Cultivar registration at the Arnold Arboretum 1994. Two new cultivars were registered. Malus pumila cultivar Pink Joy, first observed in 1978, is a chance seedling that produces variegated flowers in which the petals are pink and white, with the lower surfaces more pink than the upper ones. Ulmus d...

Goyal, R.; Das, R.; Bambery, P.; Garewal, G., 2008: Serum transferrin receptor-ferritin index shows concomitant iron deficiency anemia and anemia of chronic disease is common in patients with rheumatoid arthritis in north India. Anemia is a frequent cause of morbidity in patients with rheumatoid arthritis (RA). We studied the prevalence of anemia of chronic disorders (ACD) and ACD with coexistent iron deficiency anemia (IDA) in patients with RA using sTfR/log ferritin rat...

Taranenko, L.D.; Tamarkina, A.S.; Oberemchenko, A.I., 1969: Irrigoscopy in the diagnosis of atypical form of appendicitis. Klinicheskaia Khirurgiia 10: 73-74

L.M.rca, E..; Soriano, P.., 2000: Spilotes pullatus (tigra cazadora, tropical rat snake). Herpetological Review. March; 311: 57-58

Fu, R.; Cotten, M.; Cross, T.A., 2000: Inter- and intramolecular distance measurements by solid-state MAS NMR: determination of gramicidin A channel dimer structure in hydrated phospholipid bilayers. Distance constraints are an important complement to orientational constraints. While a high-resolution monomer structure of the ion channel forming polypeptide, gramicidin A, has been solved with 120 orientational constraints, the precise geometry...

Klimontovich, Y.L.; Vasil'ev, V.A., 1997: Determination of the relative degree of order in a chain of diffusion-linked self-oscillators simulating peristalsis of thin tubules according to the S-theorem criterion. The criterion of the relative degree of order (RDO) on the basis of the S-theorem for a non-equilibrium system is applied to the analysis of the state of a chain of diffusion-linked self-oscillators, a system which models the peristalsis of thin t...

Nozais, J. P., 1992: A new case of probable cross resistance to mefloquine and halofantrine in Plasmodium falciparum from Sierra-Leone. A French tourist suffered an attack of malaria, 2 days after taking the last prophylactic dose of mefloquine (250 mg) on his return to France from Sierra Leone. Blood tests were positive for P. falciparum. The patient was prescribed repeated doses...

Hannam, J.A.; Anderson, B.J.; Mahadevan, M.; Holford, N.H.G., 2015: Postoperative analgesia using diclofenac and acetaminophen in children. Diclofenac dosing in children for analgesia is currently extrapolated from adult data. Oral diclofenac 1.0 mg·kg(-1) is recommended for children aged 1-12 years. Analgesic effect from combination diclofenac/acetaminophen is unknown. Children (n...

Nogawa, K.; Kawano, S.; Kato, T.; Sakamoto, M., 1982: The prevalence of itai-itai disease on the mean cadmium concentration in rice produced by individual villages. Cd conc. in rice were employed as an index of Cd exposure. Samples of unpolished rice were collected from 727 households in 40 villages along the Cd polluted Jinzu River basin and analysed. Prevalence of itai-itai disease was used as an index of e...