EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of messenger RNA from mature skeletal and cardiac muscle


, : Preparation of messenger RNA from mature skeletal and cardiac muscle. Canadian Journal of Biochemistry 60(6): 587-592

Messenger RNA (poly(A) + RNA) has been prepared from mature skeletal muscle of adult rats by several procedures. These procedures involved oligodT-cellulose chromatography of either muscle polyribosomes (method 1), polysomal RNA isolated by CsCl gradient centrifugation of muscle polysomes (method 2), total muscle RNA isolated by CsCl gradient centrifugation (method 3), or total muscle RNA prepared by phenol extraction (method 4). Each procedure produced RNA in significant yields which sedimented in a broad band (from 4S to greater than 28S) on sodium dodecyl sulfate--sucrose gradients. In addition, poly (A) + RNA from each procedure stimulated protein synthesis in the wheat germ cell-free system. The best combination of yield and messenger activity was obtained for poly (A) + RNA prepared from polysomal RNA by method 2. This poly(A) + RNA preparation stimulated the cell-free synthesis of a number of presumptive myofibrillar proteins, including myosin heavy chain and actin, in the wheat germ system. The presence of the latter protein among the cell-free products was confirmed by DNase I affinity chromatography of appropriate reaction mixtures. Poly(A) + RNA was also isolated from rat cardiac muscle polysomal RNA by method 2. This RNA also directed the synthesis of myofibrillar proteins in the wheat germ system. The relative amounts of the proteins synthesized in the presence of skeletal and cardiac muscle poly(A) + RNA have been compared. The data indicate that the method described is suitable for the isolation of RNA with message activity from mature mammalian muscle.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006173459

PMID: 7116203

DOI: 10.1139/o82-072

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Mendonga de Oliveira, MI., 1971: ContribuiYao ao estudo da malacofauna intertidal de recifes de Arenite no nordeste Brasileiro. Arquivos Cienc Mar, 112: 83-86

Lo, C.W.; Chung, Q.Y., 1979: The sedative effect of acupuncture. Eight patients with a diagnosis of anxiety neurosis were treated with acupuncture. Of them, 6 showed good to moderate response while 2 had no change. The endorphin theory in applying acupuncture is discussed.

Imamura, Hiroyuki; Uchida, Kazuhiro; Kobata, Daikichi, 2000: Relationship of cigarette smoking with blood pressure, serum lipids and lipoproteins in young Japanese women. 1. The relationship of cigarette smoking with blood pressure and serum lipids and lipoproteins was studied in 1062 young Japanese women aged 20-39 years of age. 2. After adjusting for age, body mass index (BMI), alcohol intake and physical activit...

Travis, H. J., 2005: The effect of Eastern tent caterpillar (Malacosoma americanum) infestation on fall webworm (Hyphantria cunea) selection of black cherry (Prunus serotina) as a host tree. A study was undertaken in 2001 to determine whether the presence of Eastern tent caterpillars (M. americanum) on black cherry (P. serotina) affects selection of the same tree by fall webworms (H. cunea) in the same season. Five study sites were se...

Philipson W.R., 1987: The treatment of isolated genera. Problems presented by [plant] genera, or small groups of genera, which have been given family rank are reviewed, and the genera are divided into a number of categories according to the closeness of their affinity to other genera or families. Satel...

Cordeiro, James-(Jay), R., 2007: Confirmed absence of a relict population of Gonidea angulata (Lea, 1838) (Mollusca : Bivalvia : Unionidae) in Colorado. The diversity of freshwater mussels in Colorado is low compared to other regions of the U.S.A., with only three extant species (down from seven a century ago). The western ridged mussel (Gonidea angulata) occurs in Pacific Coast drainages from Br...

Slepushkina, I. I.; Litvinova-Yu, A.; Glushchenko, A. G.; Emishyan, N. D.; Zherdienko, L. G.; Kareva, V. A.; Ivakhno, A. P.; Mikhailova, A. V., 1977: Teaching at a preparatory college department as a means of adapting the student body to study loads. Study at a preparatory college contributes to improving the functional state of the CNS, the function of the higher nervous activity, and metention of a much higher level of working capacity during the following stages of studies. It provides a gr...

Chen Xuejun; Chen Zhihua; Chen Xianhua; Hu Cheng, 2000: Fuzzy-hierarchy prediction of karst collapse in western Guilin City, Guangxi, China. The karst collapse disaster in the west area of Guilin city is very typical and serious. Based on the clarification of the geological background and the analysis of a good deal of data, this paper specifies the influencing factors and degrees of k...

Hackman Robert J., 1967: Time, shadows, terrain, and photointerpretation. U S

Smith H.W., 1978: Effects of set on interpretation by subject of placebo marihuana effects. Social Science & Medicine Part A Medical Psychology & Medical Sociology 12(2): 107-110