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Preparation of metaphase chromatin of Physarum polycephalum without the loss of repressed RNA synthesis


Biochemical and Biophysical Research Communications 104(2): 657-663
Preparation of metaphase chromatin of Physarum polycephalum without the loss of repressed RNA synthesis
A novel method for the preparation of intact chromatin from the slime mold P. polycephalum which retains the in vivo property of RNA synthesis is described. Preparations from G2-cells were highly active, while those from metaphase-cells were inactive. The plasmodial cells were disrupted by gentle homogenization on a polyethylene sieve in a neural isotonic sucrose medium containing Mg++, deoxycholate and EGTA, Ca++-chelating agent. The nuclei were lysed in a hypotonic buffer without use of EDTA and chromatin was precipitated by centrifugation after addition of Mg++.


Accession: 006173466

PMID: 7073706



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