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Preparation of metaphase chromatin of Physarum polycephalum without the loss of repressed RNA synthesis


, : Preparation of metaphase chromatin of Physarum polycephalum without the loss of repressed RNA synthesis. Biochemical and Biophysical Research Communications 104(2): 657-663

A novel method for the preparation of intact chromatin from the slime mold P. polycephalum which retains the in vivo property of RNA synthesis is described. Preparations from G2-cells were highly active, while those from metaphase-cells were inactive. The plasmodial cells were disrupted by gentle homogenization on a polyethylene sieve in a neural isotonic sucrose medium containing Mg++, deoxycholate and EGTA, Ca++-chelating agent. The nuclei were lysed in a hypotonic buffer without use of EDTA and chromatin was precipitated by centrifugation after addition of Mg++.

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Accession: 006173466

PMID: 7073706

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Perez-Castillo, A.; Bernal, J.; Ferreiro, B.; Pans, T., 1985: The early ontogenesis of thyroid hormone receptor in the rat fetus. We have determined the concentration of thyroid hormone receptor binding sites in nuclear extracts derived from rat fetal organs throughout gestation and the postnatal period. Before day 14 of gestation nuclear extracts were obtained from whole fe...

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Ulmer K.M.; Gomez R.F.; Sinskey A.J., 1979: Ionizing radiation damage to the folded chromosome of escherichia coli k 12 repair of double strand breaks in dna. The extremely gentle lysis and unfolding procedures that were developed for the isolation of nucleoid DNA yield undamaged, replicating genomes, permitting direct measurement of the formation and repair of DNA double-strand breaks at biologically s...