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Preparation of modified schiff reagents their shelf life and cytochemical properties


, : Preparation of modified schiff reagents their shelf life and cytochemical properties. Microscopica Acta 82(4): 373-378

New methods are described for the preparation of a modified Schiff reagent which is usually prepared with hydrochloric or sulfuric acid in conjunction with potassium metabisulphite. The novelty of the methods lies in the fact that the hydrochloric acid of the conventional Schiff reagent is replaced by organic acid, such as lactic, along with the usual potassium metabisulfite enabling the reagents to have longer shelf-life. A new method for the preparation of Schiff reagent is presented in which this reagent contains 5 ml of N HCl, instead of 10 ml/100 ml of the dye solution. These Schiff reagents that were fortified with glycine show a much longer shelf-life as compared with that of the conventional Schiff reagent which is usually 1-2 mo. The role of pH in these new modified Schiff reagents, causing an increase in the intensity of nuclear coloration following Feulgen procedure, as well as increasing their shelf-life, is discussed.

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