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Preparation of mono specific anti serum to lupine nodule glutamate dehydrogenase ec

, : Preparation of mono specific anti serum to lupine nodule glutamate dehydrogenase ec Australian Journal of Biological Sciences 34(2): 161-170

Immunization of rabbits, using biochemically homogeneous glutamate dehydrogenase [isolated from lupin nodules, inoculated with Rhizobium lupini] proved to be unsuitable to produce monospecific antiserum. The presence of traces of contaminating immunogen (undetected by physicochemical methods) induced the production of other antibodies. Procedures for rigorously establishing antisera monospecificity and a technique for preparation of monospecific antiserum, using immunologically impure antigens, are described.

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