+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of monoclonal antibodies to the avian progesterone receptor

, : Preparation of monoclonal antibodies to the avian progesterone receptor. Endocrinology 119(4): 1549-1557

In an effort to obtain additional probes for analysis of the avian progesterone receptor, this receptor was isolated and used to prepare several monoclonal antibodies. Progesterone receptor purified from oviduct cytosol by chromatography on deoxycorticosterone-Separose and heparin-agarose was used as the immunizing antigen. Twenty-nine hybridoma cultures which tested positive in an enzyme-linked immunosorbent assay against the receptor preparation were subcloned resulting in establishment of 12 stable cell lines. Of these, 5 produced antibodies capable of complexing receptor-bound progesterone from cytosol as measured by adsorption of receptor-antibody complexes onto antimouse immunoglobulin G-agarose. Each was used to generate ascites and the purified antibodies were designated .alpha.PRL 6, 11, 13, 16, and 22. In addition to precipitating receptor-bound progesterone from cytosol, the antibodies were also effective in increasing the sedimentation velocity of progesterone receptor centrifugated on glycerol gradients, and recognizing receptor proteins that were resolved by denaturing gel electrophoresis and transferred to nitrocellulose (Western blots). Immunoisolation of receptor was also demonstrated using receptor labeled covalently with the synthetic progestin, R5020. The antibodies were specific for progesterone receptor and did not cross-react with estrogen receptor from the oviduct or glucocorticoid receptor from chick liver. Two antibodies, .alpha.PRL 6 and .alpha.PR 22, also recognized some mammalian forms of the progesterone receptor. Both antibodies reacted with progesterone receptor from the rabbit uterus and .alpha.PR 6 recognized human progesterone receptor. Four of the antibodies recognized both A and B forms of the avian receptor while .alpha.PR6 was specific for the B form.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006173514

PMID: 2428599

DOI: 10.1210/endo-119-4-1549

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Ranft, K.; Reuter, H., 1996: Hodgkin's disease after splenic immunocytoma: Rare manifestation of a dual lymphoma. Background: Communications concerning Hodgkin's disease (HD) after non-Hodgkin's lymphoma (NHL) are rather rare, whereas NHL after HD represents a common form of dual lymphoma. Patient: A 68-year-old woman developed nodular sclerosing HD...

Lasso, A; Neogrady, Zs, 1980: Antiobic resistance tests under practical conditions and some experiences on the aimed therapy. Magyar allatorvosok lapja 35(4): 262-263

Shvayko, I. I., 1957: Influence of salts of potassium and calcium on the function of the thyroid gland in rats. Female rats 6-8 months old were sustained on a full-value ration. Some of the rats were given additional food: 40-80 mg/100 g Ca, or 20 mg/100 g K. The absorbtion of I131 and average content in the thyroid gland of I served as an indicator of the...

T. P. Tourova; O. L. Kovaleva; B. K. Bumazhkin; E. O. Patutina; B. B. Kuznetsov; I. A. Bryantseva; V. M. Gorlenko; D. Yu. Sorokin, 2011: Application of ribulose-1,5-bisphosphate carboxylase/oxygenase genes as molecular markers for assessment of the diversity of autotrophic microbial communities inhabiting the upper sediment horizons of the saline and soda lakes of the Kulunda Steppe. The genes encoding the key metabolic reactions are often used as functional markers for phylogenetic analysis and microbial ecology studies. The composition and structure of the genes encoding ribulose-1,5-bisphosphate carboxylase (RuBisCO) of var...

Liou MinLi; Guo JieWei; Wu ShuTu, 2002: Combining ability analysis of yield components in okra. Combining ability and heterosis for fruit yield and associated traits were studied in a 6x6 diallel crosses of okra (Abelmoschus esculentus). Days to flowering, number of fruits per plant, yield, fruit diameter and fruit weight were controlled by...

Kudo, Yasusei; Kitajima, Shojiro; Sato, Sunao; Ogawa, Ikuko; Miyauchi, Mutsumi; Takata, Takashi, 2002: Transfection of p27Kip1 threonine residue 187 mutant type gene, which is not influenced by ubiquitin-mediated degradation, induces cell cycle arrest in oral squamous cell carcinoma cells. Objective: It is well known that reduction of the cyclin-dependent kinase inhibitor p27Kip1 protein correlates with the malignant behavior of various cancers including oral squamous cell carcinoma (OSCC). The loss of p27Kip1 protein is suggested t...

Hossain, M. S.; Ferduos, A. J.; Hasan, C. M., 1995: Alkaloids from the stem bark of Lesmos longiflorus. Fitoterapia 66(5): 463-464

Walsh, V.; Somody, L.; Farrell, A.; Zhang, B.; Brown, J.; Pritchard, C.; Vincent, M.; Samani, N.J., 2003: Analysis of the role of the SA gene in blood pressure regulation by gene targeting. The SA gene is expressed in the proximal tubule of the kidney and may be involved in blood pressure (BP) regulation. However, direct evidence for this is lacking. We constructed and analyzed an SA-null mouse in which exons 2 and 3 of the. SA gene...

Bouaziz, H.; Tong, C.; Eisenach, J. C., 1994: Systemic opioids stimulate norepinephrine release from sheep spinal cord An in vivo microdialysis study. Anesthesiology (Hagerstown) 81(3A): A988

Sidal, Ugur; Kolankaya, Nazif, 1995: The effects of silver sulfadiazine and cephalosporins on the growth of Pseudomonas aeruginosa. P. aeruginosa is the most common microorganism isolated from burn wounds. This microorganism not only prevents the healing of wounds but may also cause sepsis. Here the effect on the in vitro growth of P. aeruginosa with various cephalosporin anti...