EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of mouse monoclonal antibodies and evidence for a host immune response to the preacetabular gland proteinase of Schistosoma mansoni cercariae


American Journal of Tropical Medicine and Hygiene 35(3): 536-543
Preparation of mouse monoclonal antibodies and evidence for a host immune response to the preacetabular gland proteinase of Schistosoma mansoni cercariae
Forty-six monoclonal antibodies were produced against the preacetabular gland secretions of Schistosoma mansoni cercariae by two different immunization protocols. These antibodies were of both the IgG and the IgM classes. One IgM monoclonal antibody (Ia4D6) was further characterized. It was specific to the cercarial stage of ELISA and showed specific binding to the 30,000 Mr proteinase in crude cercarial secretions by Western blot analysis. Preincubation of this antibody with purified cercarial proteinase resulted in inhibition of proteolytic activity, and it mediated complement-dependent cytotoxicity to cercariae in vitro. Immunoperoxidase staining of cercariae localized this antibody to vesicles visible within the preacetabular glands and their secretory ducts, and to secreted material. ELISA and Western blot analysis also showed that sera from infected mice and patients with schistosomiasis reacted with the cercarial proteinase. These studies demonstrate that a proteinase secreted into the host by invading cercariae is immunogenic and provide a monoclonal antibody probe for further characterization of its structure and function.


Accession: 006173535

PMID: 2422971



Related references

Purification and characterization of an elastase from the preacetabular gland secretions of schistosoma mansoni cercariae. Federation Proceedings 42(7): ABSTRACT 143, 1983

Quantitative collection and proteolytic activity of preacetabular gland enzyme (s) of cercariae of schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 27(3): 548-553, 1978

Quantitative collection and proteolytic activity of preacetabular gland enzyme(s) of cercariae of Schistosoma mansoni. American Journal of Tropical Medicine and Hygiene 27(3): 548-553, 1978

Antibodies induced in mice by a DNA-construct coding for the elastase of Schistosoma mansoni recognize the enzyme in secretions and preacetabular glands of cercariae. Parasitology 124(Pt 3): 301-306, 2002

Collection of a secreted protease from the preacetabular glands of cercariae of Schistosoma mansoni. Journal of Parasitology 59(4): 741-743, 1973

Calcium carbonate content of the preacetabular glands of Schistosoma mansoni cercariae. Journal of Parasitology 63(1): 163-165, 1977

Collection of secreted protease from the preacetabular glands of cercariae of Schistosoma mansoni. Journal of Parasitology 59(4): 741-743, 1973

Schistosoma mansoni: histological localization of gelatinase in the preacetabular glands of cercariae. Experimental Parasitology 34(3): 382-392, 1973

Schistosoma mansoni: partial characterization of enzyme(s) secreted from the preacetabular glands of cercariae. Experimental Parasitology 40(1): 33-40, 1976

Recognition of cercariae or schistosomula of schistosoma mansoni by monoclonal antibodies. Clinical Research 37(2): 443A, 1989