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Preparation of mutants of Trichoderma reesei with enhanced cellulase production

, : Preparation of mutants of Trichoderma reesei with enhanced cellulase production. Applied and Environmental Microbiology 34(6): 777-782

The development of an agar plate screening technique has allowed the isolation of a range of mutants of Trichoderma reesei capable of synthesizing cellulase under conditions of high catabolite repression. The properties of one of these mutants (NG-14) is described to illustrate the use of this technique. NG-14 produced five times the filter paper-degrading activity per ml of culture medium and twice the specific activity per mg of excreted protein in submerged culture when compared with the best existing mutant, QM9414. NG-14 also showed enhanced endo-beta-glucanase and beta-glucosidase production. Although these mutants were isolated as cellulase producers in the presence of 5% glycerol on agar plates, in similar liquid medium, NG-14 exhibits only partial derepression of the cellulase complex. Since the proportions of filter paper activity, endo-beta-glucanase, and cellobiase were not the same in mutants NG-14 and QM9414, and the yields of each enzyme under conditions repressive for cellulase synthesis were different, differential control of each enzyme of the cellulase complex is implied. These initial results suggest that the selective technique for isolating hyper-cellulase-producing mutants of Trichoderma will be of considerable use in the development of commercially useful cellulolytic strains.

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Accession: 006173541

PMID: 413483

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Related references

Montenecourt, B.; Eveleigh, D., 1977: Preparation of mutants of Trichoderma reesei with enhanced cellulase

Tangnu S.K.; Blanch H.W.; Wilke C.R., 1981: Enhanced production of cellulase hemi cellulase and beta glucosidase by trichoderma reesei rut c 30. The production of cellulases and hemicellulases was studied with T. reesei Rut C-30. This organism produced, together with high cellulase activities, considerable amounts of xylanases and .beta.-glucosidase. Three cellulose concentrations (1, 2.5...

Gadgil, N.J.; Daginawala, H.F.; Chakrabarti, T.; Khanna, P., 1995: Enhanced cellulase production by a mutant of Trichoderma reesei. The need for renewable energy resources has focused attention on the renewable polysaccharide cellulose, which can be enzymatically hydrolyzed to yield sugars. Trichoderma reesei QM9414, a cellulolytic microorganism, was subjected to mutagenesis u...

Hendy, N.A.; Wilke, C.R.; Blanch, H.W., 1984: Enhanced cellulase ec production in fed batch culture of trichoderma reesei c 30. The use of a fed-batch cultivation of the fungus T. reesei (C30) allows cellulase [see 1,4-(1,3;1,4)-.beta.-D-glucan 4-glucanohydrolase, EC] production to occur under optimum conditions. Extremely high enzyme levels of 26 U ml-1 at product...

Ike, M.; Park, J-yil.; Tabuse, M.; Tokuyasu, K., 2010: Cellulase production on glucose-based media by the UV-irradiated mutants of Trichoderma reesei. From 22,791 mutants of a cellulase hyper-producing strain of Trichoderma reesei (Hypocrea jecorina), ATCC66589, as the parent, we selected two mutants, M2-1 and M3-1, that produce cellulases in media containing both cellulose and glucose. The muta...

Fang, H.; Xia, L., 2014: High activity cellulase production by recombinant Trichoderma reesei ZU-02 with the enhanced cellobiohydrolase production. The cbh1 strong promoter was employed to over-express the cbh2 gene for enhancing cellobiohydrolase (CBH) production in Trichoderma reesei because CBH II component has higher specific activity than CBH I and is an important component in cellulase....

Kawamori, M.; Ado, Y.; Takasawa, S., 1986: Preparation and application of Trichoderma reesei mutants with enhanced b-glucosidase. Agricultural and Biological Chemistry 50: 77-82

Bailey M.J.; Nevalainen K.M.H., 1981: Induction isolation and testing of stable trichoderma reesei mutants with improved production of solubilizing cellulase. A mutant strain of the fungus T. reesei giving high cellulase production but having poor stability was further mutagenized to produce strains in which good production properties were retained and stability was greatly improved. The increase in the...

Rakshit S.K.; Sahai V., 1991: Optimal control strategy for the enhanced production of cellulase enzyme using the new mutant trichoderma reesei e 12. Cellulase enzyme production was enhanced using the mutant strain Trichoderma reesei E-12, which was shown to be partially resistant to catabolite repression. An optimal profile for pH, which was found to be the critical environmental parameter, wa...

Acebal C.; Castillon M.P.; Estrada P.; Mata I.; Costa E.; Aguado J.; Romero D.; Jimenez F., 1986: Enhanced cellulase production from trichoderma reesei qm 9414 on physically treated wheat straw. Trichoderma reesei QM 9414 was grown on wheat straw as the sole carbon source. The straw was pretreated by physical and chemical methods. The particle size of straw was less than 0.177 mm. Growth of T. reesei QM 9414 was maximal with alkali-pretre...