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Preparation of n chloro 5 dimethylamino naphthalene 1 sulfonamide and its application as a fluorescence reagent for the detection of phenyl thio hydantoin amino acids


, : Preparation of n chloro 5 dimethylamino naphthalene 1 sulfonamide and its application as a fluorescence reagent for the detection of phenyl thio hydantoin amino acids. Journal of Chromatography 205(2): 349-354

N-Chloro-5-dimethylaminonaphthalene-1-sulfonamide (NCDA) was prepared by the action of sodium hypochlorite on dansyl. NCDA is non-fluorescent, but developed intense fluorescence on reaction with phenylthiohydantoinamino acid. About 40-60 pmol of phenylthiohydantoinamino acids were detected on thin-layer plates. NCDA was effective in the determination of phenylthiohydantoinamino acids on TLC.

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