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Preparation of oxygen evolving photosystem ii sub chloroplasts from spinach spinacia oleracea cultivar world best



Preparation of oxygen evolving photosystem ii sub chloroplasts from spinach spinacia oleracea cultivar world best



Biochimica et Biophysica Acta 679(2): 347-350



An O2-evolving photosystem II subchloroplast preparation was obtained from spinach chloroplasts, using low concentrations of digitonin and Triton X-100. The preparation had an O2 evolution activity equivalent to 20% of the uncoupled rate of fresh broken chloroplasts, but had no significant photosystem-I-dependent O2 uptake activity. The preparation had a chlorophyll [Chl] a/b ratio of 1.9 and a P-700/Chl ratio of 1/2400. Absorption spectra at room temperature and fluorescence emission spectra of Chl at 77.degree. K, suggested a significant decrease in photosystem I antenna chlorophylls in the O2-evolving photosystem II preparation.

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Accession: 006173629

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