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Preparation of parietal peritoneum for measurements of in vitro permeability


Artificial Organs 3(3): 219-223
Preparation of parietal peritoneum for measurements of in vitro permeability
An experimental method for in vitro studies of permeability of parietal peritoneal membrane was developed in rats. Na and K fluxes across the peritoneum lining 2 different regions (the diaphragm and the anterior abdominal wall) were estimated and electrical resistance of the isolated membranes was determined. A significant reciprocal correlation was observed between the 2 indices. Quantitative differences between the 2 types of membranes were found, the diaphragmatic region being more permeable than the other. A monolayer of mesothelial cells covering the membranes is partially responsible for these differences. The parietal peritoneum was less permeable than visceral peritoneum (mesentery) studied under identical conditions.

Accession: 006173638

PMID: 161166

DOI: 10.1111/j.1525-1594.1979.tb01051.x

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