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Preparation of penta fluorobenzyl esters of arachidonic acid lip oxygenase metabolites analysis by gas chromatography and negative ion chemical ionization mass spectrometry

, : Preparation of penta fluorobenzyl esters of arachidonic acid lip oxygenase metabolites analysis by gas chromatography and negative ion chemical ionization mass spectrometry. Journal of Chromatography Biomedical Applications 305(1): 3-12

5-Hydroxyeicosatetraenoic acid (5-HETE) and leukotriene B4 possess potent chemotactic and chemokinetic properties. Because of the very low concentrations found in certain biological systems, negative-ion chemical ionization mass spectrometry was investigated as a potential assay method for detecting these compounds. A facile derivatization to form the pentafluorobenzyl esters, and clean up are reported for these compounds at the 15-ng level. Gas chromatographic properties, negative-ion chemical ionization mass spectra and positive-ion electron impact spectra are reported for the pentafluorobenzyl ester, trimethylsilyl ether derivatives of 5-HETE and leukotriene B4 isomers.

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