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Preparation of penta fluorobenzyl esters of arachidonic acid lip oxygenase metabolites analysis by gas chromatography and negative ion chemical ionization mass spectrometry


, : Preparation of penta fluorobenzyl esters of arachidonic acid lip oxygenase metabolites analysis by gas chromatography and negative ion chemical ionization mass spectrometry. Journal of Chromatography Biomedical Applications 305(1): 3-12

5-Hydroxyeicosatetraenoic acid (5-HETE) and leukotriene B4 possess potent chemotactic and chemokinetic properties. Because of the very low concentrations found in certain biological systems, negative-ion chemical ionization mass spectrometry was investigated as a potential assay method for detecting these compounds. A facile derivatization to form the pentafluorobenzyl esters, and clean up are reported for these compounds at the 15-ng level. Gas chromatographic properties, negative-ion chemical ionization mass spectra and positive-ion electron impact spectra are reported for the pentafluorobenzyl ester, trimethylsilyl ether derivatives of 5-HETE and leukotriene B4 isomers.

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Panfil Kuncewicz, H.; Kuncewicz, A.; Gierszewski, M.; Rogowski, L., 1992: Functional properties of high-protein milk concentrates produced by ultrafiltration. Dried milk was manufactured from skim milk concentrated wholly or partially by ultrafiltration (at 45 degrees C, using Romicon equipment with type PM-50 membranes) to 15, 19 or 23% DM; part of each batch was further concentrated by evaporation at...

Pflumio F.; Fonteneau P.; Gaveriaux C.; Cammisuli S.; Loor F., 1989: The c57bl 6 nude beige mouse a model of combined t cell and nk effector cell immunodeficiency. This article describes the construction and establishment of a double congenic nude, beige C57BL/6 (B6 nu, bg) mouse strain. The mice do not show higher fragility than C57BL/6 nude mice and the double congenic strain can be maintained under conven...

Das-Neves, Fabricia-Junqueira; Mattos, Ines-Echenique; Koifman, Rosalina-Jorge, 2005: Mortalidade por cancer de colon e reto nas capitais Brasileiras no periodo 1980-1997. Background - In Brazil, colorectal tumors are among the five more important sites of neoplasms, for both sexes, in terms of mortality. The etiology of colon and rectal cancer is complex and some of the factors involved in its genesis are related t...

Menefee, B. E., 1980: Bovine immunoglobulins: the relationship of pathogen specific antibodies and the immunoglobulin pool during the acquisition of passive immunity by the calf. In a group of 20 dam-calf pairs, primiparous cows had significantly lower serum IgG at parturition than multiparous cows but there were no significant differences in IgG, IgA or IgM within colostrum or calf serum samples. Immunoglobulin concn. in...

Gong, Xiang-Qun; Shao, Qing; Lounsbury, Crystal-S; Bai, Donglin; Laird, Dale, W., 2006: Functional Characterization of a GJA1 Frameshift Mutation Causing Oculodentodigital Dysplasia and Palmoplantar Keratoderma. A frameshift mutation generated from a dinucleotide deletion (780-781del) in the GJA1 gene encoding Cx43 results in a frameshift yielding 46 aberrant amino acids after residue 259 and a shortened protein of 305 residues compared with the 382 in wi...

LiYuMei; JiangYunTian; SunZhiHui, 2008: Uniform design for optimizating regeneration shoots directly from tender leaves and plant regeneration system of Rhododendron chrysanthum Pall. Objective: The experimental was aimed to screen the optimum regeneration shoot induction media and rooting media for tender leaves of <i>Rhododendron chrysanthum</i> Pall. Method: The tender leaves of <i>Rhododendron chrysanthum&...

Pond, Wg; Snyder, W; Snook, Jt; Walker, Ef; Mcneill, Da; Stillings, Br, 1971: Relative utilization of casein, fish protein concentrate and isolated soybean protein for growth and pancreatic enxyme regeneration of the protein-calorie malnourished baby pig. Journal of nutrition: 101 (9) 1193-1200

Hendrickx Michel E., 2012: Distribution and abundance of the pelagic processid, processa pippinae wicksten mendez, 1985 decapoda, caridea, processidae, collected during the talud xiv cruise in the gulf of california,; mexico, and description of a new genus. The pelagic processid, Processa pippinae Wicksten&Mendez, 1985, has been reported previously as an endemic species in the northern Gulf of California, Mexico. Additional material collected accidentally in a benthic sledge and with a mid-water...

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