EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of penta fluorobenzyl esters of arachidonic acid lip oxygenase metabolites analysis by gas chromatography and negative ion chemical ionization mass spectrometry


Journal of Chromatography Biomedical Applications 305(1): 3-12
Preparation of penta fluorobenzyl esters of arachidonic acid lip oxygenase metabolites analysis by gas chromatography and negative ion chemical ionization mass spectrometry
5-Hydroxyeicosatetraenoic acid (5-HETE) and leukotriene B4 possess potent chemotactic and chemokinetic properties. Because of the very low concentrations found in certain biological systems, negative-ion chemical ionization mass spectrometry was investigated as a potential assay method for detecting these compounds. A facile derivatization to form the pentafluorobenzyl esters, and clean up are reported for these compounds at the 15-ng level. Gas chromatographic properties, negative-ion chemical ionization mass spectra and positive-ion electron impact spectra are reported for the pentafluorobenzyl ester, trimethylsilyl ether derivatives of 5-HETE and leukotriene B4 isomers.


Accession: 006173648



Related references

Stable isotope labelled 5-lipoxygenase metabolites of arachidonic acid: analysis by negative ion chemical ionization mass spectrometry. Prostaglandins, Leukotrienes, and Medicine 13(1): 1-8, 1984

Negative ion chemical ionization gas chromatography/mass spectrometry of valproic acid metabolites. Biomedical & Environmental Mass Spectrometry 18(10): 918-926, 1989

Determination of delta 9 tetra hydro cannabinol 11 oic acid in urine as its penta fluoro propyl derivative penta fluoro propionyl derivative by gas chromatography mass spectrometry utilizing negative ion chemical ionization. Journal of Analytical Toxicology 7(4): 198-202, 1983

Analysis of urinary metabolites of thromboxane and prostacyclin by negative-ion chemical-ionization gas chromatography/mass spectrometry. Advances in Prostaglandin, Thromboxane, and Leukotriene Research 15: 87-90, 1985

Gas chromatography/negative ion chemical ionization mass spectrometry and liquid chromatography/electrospray ionization tandem mass spectrometry quantitative profiling of N-acetylcysteine conjugates of valproic acid in urine: application in drug metabolism studies in humans. Journal of Mass Spectrometry 35(6): 698-704, 2000

Biosynthesis of arachidonic acid metabolites in Limulus polyphemus amebocytes: Analysis by liquid chromatography-electrospray ionization mass spectrometry. Biochimica et Biophysica Acta 1303(2): 127-136, 1996

Isotopic determination of organic keto acid pentafluorobenzyl esters in biological fluids by negative chemical ionization gas chromatography/mass spectrometry. Analytical Chemistry 63(9): 919-923, 1991

Determination of anabolic steroids by gas chromatography/negative-ion chemical ionization mass spectrometry and gas chromatography/negative-ion chemical ionization tandem mass spectrometry with heptafluorobutyric anhydride derivatization. Rapid Communications in Mass Spectrometry 13(5): 376-380, 1999

Determination of four anabolic steroid metabolites by gas chromatography/mass spectrometry with negative ion chemical ionization and tandem mass spectrometry. Rapid Communications in Mass Spectrometry 12(22): 1749-1755, 1998

Analysis of biogenic amines and their metabolites in biological tissues and fluids by gas chromatography-negative ion chemical ionization mass spectrometry (GC-NICIMS). Journal of Pharmaceutical and Biomedical Analysis 8(8-12): 899-904, 1990