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Preparation of per oxidase anti per oxidase complexes for immuno histological labeling of mono clonal antibodies


Journal of Histochemistry & Cytochemistry 30(11): 1114-1122
Preparation of per oxidase anti per oxidase complexes for immuno histological labeling of mono clonal antibodies
The production of mouse peroxidase:antiperoxidase (PAP) complexes suitable for immunohistological use in conjunction with monoclonal antibodies is described. Three approaches were explored: production of conventional polyclonal PAP complexes; conversion of rabbit PAP to pseudo-mouse PAP by incubation with monoclonal mouse anti-rabbit Ig; formation of PAP complexes from monoclonal mouse antiperoxidase. PAP complexes prepared by the latter technique gave the best immunohistological labeling reactions, being stable on storage and compatible with a wide range of human monoclonal antibodies. Gel filtration revealed that monoclonal PAP is of lower MW than conventional PAP complexes (fulfilling theoretical predictions based on the monospecificity of monoclonal antibodies).


Accession: 006173652



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