+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of per oxidase anti per oxidase complexes for immuno histological labeling of mono clonal antibodies

, : Preparation of per oxidase anti per oxidase complexes for immuno histological labeling of mono clonal antibodies. Journal of Histochemistry & Cytochemistry 30(11): 1114-1122

The production of mouse peroxidase:antiperoxidase (PAP) complexes suitable for immunohistological use in conjunction with monoclonal antibodies is described. Three approaches were explored: production of conventional polyclonal PAP complexes; conversion of rabbit PAP to pseudo-mouse PAP by incubation with monoclonal mouse anti-rabbit Ig; formation of PAP complexes from monoclonal mouse antiperoxidase. PAP complexes prepared by the latter technique gave the best immunohistological labeling reactions, being stable on storage and compatible with a wide range of human monoclonal antibodies. Gel filtration revealed that monoclonal PAP is of lower MW than conventional PAP complexes (fulfilling theoretical predictions based on the monospecificity of monoclonal antibodies).

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 006173652

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Hallenstvet, M.; Liaaen Jensen, S., 1979: Carotenoids from soft corals. Biochemical Systematics and Ecology 7(2): 171-172

Klang, F.; Eko, P. M., 1999: Tree properties and yield of Picea abies planted in shelterwoods. Tree properties affecting timber quality and yield were examined in 6 stands of Norway spruce (Picea abies) planted in shelterwoods in southern Sweden. For each stand a pairwise comparison was made with trees planted in the open. The shelterwoods...

Fourmanoir, P., 1961: Liste complementaire des Poissons du Canal de Mozambique. Memoires de l'Institut Scientifique de Madagascar, 4F 83-107

Riley, Simon C.; Wong, Evelyn; Findlay, Jock K.; Salamonsen, Lois A., 1995: Localization of neutral endopeptidase in the ovine uterus and conceptus during the oestrous cycle and early pregnancy. Neutral endopeptidase (NEP; EC, an enzyme which metabolizes several peptides (including oxytocin and endothelins) implicated in the control of uterine function, was found to be localized in the ovine uterus throughout the oestrous cycle...

Edens N.K.; Friedman M.I., 1984: Response of normal and diabetic rats to increasing dietary medium chain tri glyceride content. Replacing dietary long-chain triglycerides (LCT) with medium-chain triglycerides (MCT) has not previously been shown to produce reliable changes in caloric intake. Diabetic rats are particularly sensitive to changes in the fat content of their die...

Nouzarede, M.; Renaud-Mornant, J., 1965: De la representation graphique des populations heterogenes et de leurs variations numeriques. Vie et Milieu, 16B1: 423-439

Krause, N., 1990: Short synthesis of dextro 4 8 dimethyl 1 oxaspiro 4.5 deca 3 7 dien 2 one andirolactone using the propynoate cuprate method. Racemic 4,8-dimethyl-1-oxaspiro[4.5]deca-3,7-dien-2-one (andirolactone) (1) was synthesized in two steps with 58% overall yield, starting from 4-methyl-3-cyclohexenone (2) and methyl propynoate (3).

Tallitsch R.B., 1980: Stimulation by aldo sterone of the sodium efflux in barnacle balanus nubilus muscle fibers lack of a gluco corticoid effect. IRCS (International Research Communications System) Medical Science Library Compendium 8(12): 875-876

Curelariu, N; Georgescu, G1; Diaconescu, S1; Balan, So, 1982: Stocking of cattle farms--a major technological factor in achieving high production Romania.1. Lucrari stiintifice Seria D: Zootehnie5(25): 75-79

Bagnall, R. S., 1934: On two new British species of Odontothrips Uz. (Thysanoptera). Entomologist's Monthly Magazine, 70: 59-60