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Preparation of poly adenylic acid binding proteins from endoplasmic reticulum containing subfractions of rat liver cells and their use in messenger rna purification


Molecular and Cellular Biochemistry 15(1): 67-71
Preparation of poly adenylic acid binding proteins from endoplasmic reticulum containing subfractions of rat liver cells and their use in messenger rna purification
Approximately 2% of the proteins solubilized from rat liver microsomes or rapidly sedimenting endoplasmic reticulum (RS-ER) adsorbed to poly(A)-Sepharose. The adsorption was selective for a few proteins, and proteins of different apparent MW adsorbed from RS-ER and the microsomes. The proteins from RS-ER with affinity for poly(A) were coupled to Sepharose and used for the purification of mRNA from rabbit mammary glands. A portion of the RNA which did not adsorb to poly(U)-Sepharose adsorbed to protein-Sepharose and was active in a cell-free protein synthesis system.


Accession: 006173688



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