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Preparation of polyvalent antibodies for use in simultaneous multiple drug radio immunoassay


Journal of Analytical Toxicology 1(1): 14-19
Preparation of polyvalent antibodies for use in simultaneous multiple drug radio immunoassay
Antisera against several drugs were prepared in the same animal and utilized in a polyvalent radioimmunoassay (RIA) capable of screening biological specimens for a number of drugs simultaneously in a single assay. A group of rabbits were immunized sequentially or simultaneously with drug protein conjugates of compounds related to morphine, barbiturate, cocaine and diazepam. The polyvalent antisera thus obtained were capable of binding opiates, barbiturates, cocaine and diazepam and their metabolites individually and/or in combination. The antibody titers for the drugs ranged 1:5-1:200. Human urines (1000) randomly selected from drug abuse clinics were screened by the polyvalent RIA, and results were compared with single drug RIA, Enzyme Multiplied Immunoassay Technique (EMIT) and TLC tests. When compared with the results from individual RIA, the polyvalent RIA had .apprx. 4.3% false positive and no false negative results. Compared with EMIT and TLC there was significant agreement of positive and negative specimens. These data represent the 1st preparation of a polyvalent antiserum and its application in simultaneous dual, triple and quadruple radioimmunoassays. The preparation of polyvalent antisera requires fewer animals and provides more uniform antisera than the in vitro mixing of univalent antisera from many animals. Polyvalent antiserum can be used as an immunoassay exclusion test in drug screening and in forensic toxicologic analyses. These antisera can be used for univalent assays or for quantitative analyses of individual drugs under controlled conditions.


Accession: 006173701



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