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Preparation of protein hydrolysates with the use of immobilized bacterial peptide hydrolases


, : Preparation of protein hydrolysates with the use of immobilized bacterial peptide hydrolases. Antibiotiki i Meditsinskaya Biotekhnologiya 30(4): 243-249

The preparation of medical protein hydrolysates with the use of complete hydrolysis of proteins by bacterial enzyme complexes, such as protosubtilin G10x and bacterial peptidase immobilized on aminosilylated alumina, is possible. The activity, thermostability and substrate specifity of the heterogenous biocatalysts were studied. The integral kinetics of the hydrolysis of sodium caseinate and peptides included in partial acid and enzymatic casein hydrolysates and blood was investigated. The engineering approaches to mathematical simulation of the bioproteolysis kinetics and the results of their use in processing of the experimental data are discussed.

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