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Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis

Journal of General Microbiology 115(2): 517-521

Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis

Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).

Accession: 006173732

PMID: 119034

DOI: 10.1099/00221287-115-2-517

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