EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis


, : Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis. Journal of General Microbiology 115(2): 517-521

Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006173732

PMID: 119034

DOI: 10.1099/00221287-115-2-517

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Walland, A.; Hammer, R., 1997: Muscarinic contraction in isolated guinea-pig trachea and antagonism by noradrenaline. In contrast to other muscarinic agonists, WAL 2014 FU does not induce bronchospasm in laboratory animals. The present investigation was intended to test the hypothesis that this is due to a particular susceptibility of the drug's effect to an...

Matsumori K.; Taira T., 1986: Evaluation of lymphocyte stimulation test in choice of anticonvulsants. Journal of Tokyo Women's Medical College 56(9): 797-803

Sousa. O. Do C., 1970: Control of external parasites of cattle. In this discussion of tick infestation of cattle with particular reference to Mozambique, the various kinds of tick-host relationships and the harm done by ticks are briefly reviewed, and the maximum periods for which some of the commonest ticks i...

Ferrier, J.; Illeman, A.; Zakshek, E., 1985: Transient and sustained effects of hormones and calcium on membrane potential in a bone cell clone. Measurements were made of the electrophysiological and cAMP response to changes in extracellular [Ca2+] and to hormone application in a bone cell clone. Both transient and long-term electrophysiological responses were studied. An increase in extra...

Fall, M.; Nasir, O., 2010: Mechanical behaviour of the interface between cemented tailings backfill and retaining structures under shear loads. Understanding the shear behavior of the interface between cemented tailings backfill and retaining wall structures (barricades, bulkheads) is important for the optimal design of barricades or bulkheads, and for mine operators to balance strength a...

Beutler, E.; McMillan, R.; Spruce, W., 1982: The role of bone marrow transplantation in the treatment of acute leukemia in remission. Blood 59(6): 1115-1117

Vaziri, N. D.; Oveisi, Fariba; Wierszbiezki, Maryann; Shaw, Viviana; Sporty, Lawrence, D., 1993: Serum melatonin and 6-sulfatoxymelatonin in end-stage renal disease: Effect of hemodialysis. The pineal hormone melatonin plays a role in the regulation of hypothalamic-pituitary axis, target gland function, sleep pattern, mood changes, cellular immunity, antibody response, and skin pigmentation, nearly all of which are also affected by r...

Kozlovskiy Ye A., 1993: Tendencies in the development of the mineral-raw material base of the world. Geologicheskiy Zhurnal (Kiev, 1968) 1993(1): 41-44

Dore N.; Collins J P.; Mankiewicz E., 1979: A sporotrichoid like mycobacterium kansasii infection of the skin treated with minocycline hydro chloride. A sporotrichoid-like M. kansasii [human] skin infection is reported. This is the 5th reported case in the English literature of dermatological manifestations of M. kansasii infection and the 1st reported case of response to minocycline hydrochlori...

Mason, R.G.; Gilkey, J.M., 1971: A simple test for quantitation of platelet adhesion to glass. Studies in bleeder and nonbleeder subjects. Thrombosis et Diathesis Haemorrhagica 25(1): 21-29