EurekaMag.com logo
+ Site Statistics
References:
52,725,316
Abstracts:
28,411,598
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis






Journal of General Microbiology 115(2): 517-521

Preparation of protoplasts and whole cell ghosts from Mycobacterium smegmatis

Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).


Accession: 006173732

PMID: 119034

DOI: 10.1099/00221287-115-2-517



Related references

Identification of mycobacterium phlei tmc 1523 and mycobacterium smegmatis sn 40 as members of mycobacterium smegmatis and mycobacterium fortuitum respectively selective inhibition by trimethoprim. International Journal of Systematic Bacteriology 28(4): 602-604, 1978

Organization of the origins of replication of the chromosomes of Mycobacterium smegmatis, Mycobacterium leprae and Mycobacterium tuberculosis and isolation of a functional origin from M. smegmatis. Molecular Microbiology 20(2): 283-293, 1996

Inhibition of mycolic acid biosynthesis in a cell-wall preparation from Mycobacterium smegmatis by methyl 4-(2-octadecylcyclopropen-1-yl)butanoate, a structural analogue of a key precursor. Letters in Applied Microbiology 17(1): 33-36, 1993

Elemental analysis of Mycobacterium avium-, Mycobacterium tuberculosis-, and Mycobacterium smegmatis-containing phagosomes indicates pathogen-induced microenvironments within the host cell's endosomal system. Journal of Immunology 174(3): 1491-1500, 2005

Cross-resistance relationship between streptomycin and kanamycin resistances in Mycobacterium smegmatis (strain Jucho)--comparison of the development patterns of resistances to streptomycin and kanamycin among Mycobacterium tuberculosis, Mycobacterium avium complex, and Mycobacterium smegmatis. Kekkaku 65(12): 785-789, 1990

Stimulation of mycolic acid biosynthesis by incorporation of cis-tetracos-5-enoic acid in a cell-wall preparation from Mycobacterium smegmatis. Biochimica et Biophysica Acta 1167(2): 182-188, 1993

Expression of the Mycobacterium bovis P36 gene in Mycobacterium smegmatis and the baculovirus/insect cell system. Brazilian Journal of Medical and Biological Research 32(1): 29-37, 1999

Screening of a cosmid library of Mycobacterium bovis BCG in Mycobacterium smegmatis for novel T-cell stimulatory antigens. Research in Microbiology 144(5): 349-362, 1993

Comparison of the interaction between THP-1 cells and cell wall components of Mycobacterium avium ssp paratuberculosis and Mycobacterium smegmatis. 2007

Identification of bovine T cell stimulatory antigens using a cosmid library of Mycobacterium bovis in Mycobacterium smegmatis. New Zealand Veterinary Journal 43(7): 360-364, 1995