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Preparation of protoplasts from mycelium and arthroconidia of geotrichum candidum

, : Preparation of protoplasts from mycelium and arthroconidia of geotrichum candidum. Canadian Journal of Microbiology 31(2): 93-96

Protoplasts were released from mycelium and the cylindrical type of anthroconidia from G. candidum by a lytic enzyme (Novozym 234). The enzyme was unable to release protoplasts from the ellipsoidal type of arthroconidia, which reflects a difference in the cell walls of the 2 types of conidia. The conditions necessary to obtain stable protoplasts were determined. Magnesium sulfate (0.8 M) was the best osmotic stabilizer for the formation of protoplasts, whereas mannitol and magnesium sulfate could be used for regeneration. The regeneration was faster with mannitol than with magnesium sulfate.

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Accession: 006173733

DOI: 10.1139/m85-019

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Akmal'khanov, Sh., 1961: The composition of milk of Black Pied and Bushuev cows in Uzbekistan.. The composition and properties of milk of Black Pied and Bushuev cows and Black Pied-Zebu crosses were studied on an experimental farm in Uzbekistan. The average results are tabulated and differences discussed. Butter made from the milk of the dif...

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Prokhorov V.P.; Raitviir A., 1991: New or interesting species of ascobolus and saccobolus in the ussr. In the study of coprophilous Discomycetes 17 interesting species from the genera Ascobolus and Saccobolus have been found on samples of drug of different animals, on soil and on plant remains. Two of them - Ascobolus ursinus Prokhorov, sp. nov. an...

Dobarro,I.; Valladares,F.; Peco,B., 2010: Light quality and not quantity segregates germination of grazing increasers from decreasers in Mediterranean grasslands. Grassland plant species have traditionally been classified into two groups, <i>increasers</i> and <i>decreasers</i>, based on changes in their relative abundance in the presence or absence of grazing. However, the ecophysio...

Corney R.H.; Williams P., 1986: Social and familial dysfunction general practitioner attendance and psychiatric diagnosis. International Journal of Family Psychiatry 7(2): 137-148

Kessler, S.Kilaru.; McCarthy, A.; Cnaan, A.; Dlugos, D.J., 2015: Retention rates of rufinamide in pediatric epilepsy patients with and without Lennox-Gastaut Syndrome. To evaluate the effectiveness of rufinamide (RFM) in patients with Lennox-Gastaut Syndrome (LGS) compared to those with other epilepsy syndromes using time to treatment failure (retention rate) as the outcome measure. In this retrospective cohort...

Caro-Aguilar, I.; Lapp, S.; Pohl, J.; Galinski, M.R.; Moreno, A., 2005: Chimeric epitopes delivered by polymeric synthetic linear peptides induce protective immunity to malaria. Polymeric linear peptide chimaeras (LPCs) that incorporate Plasmodium vivax promiscuous T cell epitopes and the P. falciparum circumsporozoite protein B cell epitope have been shown to induce a high level of immunogenicity and overcome genetic res...

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Hino S., 1984: The validity of the estimated maximal oxygen intake by different indirect methods in middle aged and elderly men. The validity of estimated maximal O2 intake (.ovrhdot.VO2 max) from different conventional methods was compared and multiple regression equations were developed for the estimation of .ovrhdot.VO2 max at a bicycle ergometer. The subjects tested wer...

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