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Preparation of protoplasts of group H streptococci (Streptococcus sanguis)


Applied Microbiology 29(1): 90-93
Preparation of protoplasts of group H streptococci (Streptococcus sanguis)
Stable protoplasts of several strains of group H streptococci (Streptococcus sanguis) can be prepared by use of group C streptococcal phage-associated lysin in the presence of 30% raffinose. Sucrose cannot be substituted for raffinose. Protoplast formation did not require the addition of Mg2+; however, this cation enhanced their stability. Some other strains, also presumptive group H streptococci, were not sensitive to phage-associated lysin.


Accession: 006173735

PMID: 803288



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