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Preparation of pseudomonas putida protoplast like structures and their reversion into bacterial forms

, : Preparation of pseudomonas putida protoplast like structures and their reversion into bacterial forms. Mikrobiologiya 51(4): 628-631

A technique is proposed for the preparation of P. putida protoplast-like structures by treating the cells with lysozyme in a hypertonic medium containing monovalent and divalent cations. Pretreatment of the cells at the logarithmic growth phase with sucrose (0.5 M) for 90 min at pH 7.9-8.0 is a prerequisite for the formation of protoplasts. Under these conditions, the yield of protoplasts reached 99.9% of the total cell number. The protoplast-like structures are capable of reverting into the original bacterial forms in a minimal hypertonic medium containing amino acids which are components of the cell wall. The level of reversion reaches 10% for certain strains.

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