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Preparation of pure lines of thymic stromal fibroblasts and discrimination of fibroblasts and epithelium in thymic cultures

, : Preparation of pure lines of thymic stromal fibroblasts and discrimination of fibroblasts and epithelium in thymic cultures. Byulleten' Eksperimental'noi Biologii i Meditsiny 91(1): 61-64

Explantation of suspensions of the cells isolated from the guinea pig thymus by mechanical treatment leads to the formation in monolayer cultures of discrete fibroblast colonies; explanation of the cells obtained by trypsinization results in fibroblast colonies apart from foci of the epithelium. These 2 types of the cells are discriminated on the basis of their fixation with antifibronectin and antifibroblasts sera as well as by adhesiveness of epithelial cells and fibroblasts.

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