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Preparation of pure lines of thymic stromal fibroblasts and discrimination of fibroblasts and epithelium in thymic cultures


Byulleten' Eksperimental'noi Biologii i Meditsiny 91(1): 61-64
Preparation of pure lines of thymic stromal fibroblasts and discrimination of fibroblasts and epithelium in thymic cultures
Explantation of suspensions of the cells isolated from the guinea pig thymus by mechanical treatment leads to the formation in monolayer cultures of discrete fibroblast colonies; explanation of the cells obtained by trypsinization results in fibroblast colonies apart from foci of the epithelium. These 2 types of the cells are discriminated on the basis of their fixation with antifibronectin and antifibroblasts sera as well as by adhesiveness of epithelial cells and fibroblasts.


Accession: 006173743



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