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Preparation of radioiodinated insulin and tsh using 1 3 4 6 tetrachloro 3 alpha 6 alpha diphenylglycouril iodogen for radioimmunoassay


Journal of Radioanalytical & Nuclear Chemistry 116(1): 193-202
Preparation of radioiodinated insulin and tsh using 1 3 4 6 tetrachloro 3 alpha 6 alpha diphenylglycouril iodogen for radioimmunoassay
Radioiodinated insulin and thyroid stimulating hormones were prepared using 1,3,4,6-tetrachloro-3.alpha., 6.alpha.-diphenylglycouril (Iodogen). Conditions of iodination like concentration of iodogen, reaction time, etc., were optimized to get maximum yield. Stability studies of iodogen coated tubes were carried out over a period of time. The dependence of iodination yield on varying amounts of activity and protein concentration were investigated. Iodination yield over a pH was also studied. The radiolabelled hormones prepared by this method were used in radioimmunoassay and were compared with tracers prepared by the Chloramine-T method.


Accession: 006173781



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