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Preparation of raf oncogene specific antiserum with raf protein produced in escherichia coli


, : Preparation of raf oncogene specific antiserum with raf protein produced in escherichia coli. Biochimica et Biophysica Acta 949(2): 233-239

In this report we describe the expression of v-raf protein in E. coli using a tryptophan-promoter-driven expression vector and its immunological characterization by anti-peptide sera. The purified recombinant protein was used to produce raf-specific antibodies which are suitable for studying v-raf and c-raf proteins in vitro and in vivo in a variety of species ranging from mouse to man.

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