+ Translate

Preparation of retinal vessels for scanning electron microscopic examination using microdissection by ultrasonication

, : Preparation of retinal vessels for scanning electron microscopic examination using microdissection by ultrasonication. Current Eye Research 6(11): 1353-1358

A method of microdissection by ultrasonication was used to prepare blood vessels of the retina for scanning electron microscopic examination. Eye cups were treated with 2% OsO4 that contained 2% CHAPS or 1% saponin (16 hrs.), dehydrated to 100% acetone, sonicated at 80 kHz (40 min.), and further processed by conventional means. This treatment resulted in the separation of the outer and inner retina at the level of blood vessels in the outer plexiform layer. The overall pattern of the blood vessels was maintained. Venules were distinguished from capillaries. This technique may provide information that is not possible to obtain with corrosion casts or trypsin digests.

Accession: 006173814

PMID: 3427984

DOI: 10.3109/02713688708997562

Download PDF Full Text: Preparation of retinal vessels for scanning electron microscopic examination using microdissection by ultrasonication

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Related references

Low, F.N., 1989: Microdissection by ultrasonication for scanning electron microscopy. Progress in Clinical and Biological Research 295: 571-580

Rosinia F.A.; Low F.N., 1985: Microdissection by ultrasonication enzymatic degradation of rat endocardium for scanning electron microscopy. Anatomical Record 211(3): 352-353

Budras, J.; Weber, H., 2002: Electron microscopic examination of tumbled and in sauce-seasoned meat preparation: 1. Scanning electron microscopic methods (REMI). Meat preparations were examined by scanning electron microscopy (REMI). The aim of this study was to examine changes in the structure of the meat in order to determine its quality traits. This paper reports about striped porcine meat of the topsid...

Rosinia, F.A.; Low, F.N., 1986: Scanning electron microscopy of Thebesian ostia (microdissection by ultrasonication: enzymatic digestion). Thebesian vasculature provides for communication between the coronary system and the chambers of the heart. Anatomic, embryologic, physiologic, and therapeutic investigations have involved this component of cardiac anatomy from the early 18th cent...

Highison, G.J.; Low, F.N., 1982: Microdissection by ultrasonication after prolonged OsO4 fixation: a technique for scanning electron microscopy. A new method of tissue preparation for EM is described for the selective dissection of mammalian tissues. This technique, based on brittlization by prolonged osmication, dehydration to pure acetone and subsequent ultrasonication, was especially us...

Low, F.N.; McClugage, S.G., 1984: Microdissection by ultrasonication: scanning electron microscopy of the epithelial basal lamina of the alimentary canal in the rat. The epithelial basal lamina of the various parts of the alimentary canal of the rat was exposed by removal of the overlying epithelium. This was achieved by prolonged fixation in OsO4 or immersion in aqueous boric acid or both, followed by dehydra...

Faulborn, J., 1982: Combined macroscopic light microscopic scanning electron microscopic and transmission electron microscopic investigation of the vitreous body 1. an improved celloidin embedding method for histologic scanning electron microscopic and transmission electron microscopic preparation of the vitreous body. An improved celloidin embedding method for combined histological and EM examinations of the vitreous body [from pigs and humans] is presented which allows the topographical relationship between the different methods to be maintained.

Sekino, T., 1975: Scanning and transmission electron microscopic studies on the retinal vessels of spontaneously hypertensive rats (SHR). Nippon Ganka Gakkai Zasshi 79(8): 887-903

Vriend R.A.; Geissinger H.D., 1980: An improved direct inter microscopic light microscopic scanning electron microscopic transmission electron microscopic correlative procedure for the examination of mammalian skeletal muscle. The procedure for direct intermicroscopic (LM [light microscopic] .fwdarw. SEM [scanning electron microscopic] .fwdarw. TEM [transmission electron microscopic]) correlation of [mouse] muscle was modified. The major modification was the use of cryo...

Bhutto, I.A.; Amemiya, T., 1999: Effects of cilazapril on the retinal vessels in spontaneously hypertensive rats: corrosion cast and scanning electron microscopic study. The effects of the long-term oral angiotensin-converting enzyme inhibitor, cilazapril, on retinal circulation in stroke-prone spontaneously hypertensive (SHR-SP) rats were assessed by scanning electron microscopy (SEM), corrosion casts and transmi...