EurekaMag.com logo
+ Site Statistics
References:
48,253,228
Abstracts:
22,798,842
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Preparation of retinal vessels for scanning electron microscopic examination using microdissection by ultrasonication


, : Preparation of retinal vessels for scanning electron microscopic examination using microdissection by ultrasonication. Current Eye Research 6(11): 1353-1358

A method of microdissection by ultrasonication was used to prepare blood vessels of the retina for scanning electron microscopic examination. Eye cups were treated with 2% OsO4 that contained 2% CHAPS or 1% saponin (16 hrs.), dehydrated to 100% acetone, sonicated at 80 kHz (40 min.), and further processed by conventional means. This treatment resulted in the separation of the outer and inner retina at the level of blood vessels in the outer plexiform layer. The overall pattern of the blood vessels was maintained. Venules were distinguished from capillaries. This technique may provide information that is not possible to obtain with corrosion casts or trypsin digests.

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 006173814

PMID: 3427984

DOI: 10.3109/02713688708997562

PDF Full Text: Preparation of retinal vessels for scanning electron microscopic examination using microdissection by ultrasonication


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

De Carlo, J. A. I., 1938: Dos nuevas especies del genero Abedus Stal. II. Nuevas consideraciones sobre Belostoma costalimai De Carlo y Lethocerus truncatus Cummings (Hemiptera-Belostomidae). Revista de la Sociedad Entomologica Argentina, 10: 41-46

Viragh, S.; Gittenberger D.G.oot, A.C.; Poelmann, R.E.; Kalman, F., 1993: Early development of quail heart epicardium and associated vascular and glandular structures. As in the other vertebrates the epicardium of the quail embryo develops from proepicardial tissue located between the sinus horns and the liver primordium. The cuboidal cells of the coelomic lining above the proepicardium are transformed into meso...

Kondo, S.; Okabe, S., 2014: In vivo two-photon microscopy of microglia. In vivo imaging with two-photon microscopy is becoming an indispensable technique to investigate cellular and subcellular phenomenon in living tissues including the central nervous system. This microscopy enables to image dynamics of molecules, mo...

Wang WeiKung; Sung TzuLing; Lee ChunNan; Lin TsaiYu; King ChwanChuen, 2002: Sequence diversity of the capsid gene and the nonstructural gene NS2B of dengue-3 virus in vivo. Previously, we studied the envelope (E) gene of dengue virus and reported that dengue-3 virus is present as a quasispecies. To investigate the extent of intrahost sequence variation of other dengue viral genes, we examined in this study the capsid...

Nasehi, M.; Nasehi, M.; Rahmani-Nia, F.; Mirzaei, B.; Torabi-Nami, M.; Zarrindast, M-Reza., 2015: Swimming improves the emotional memory deficit by scopolamine via mu opioid receptors. The aim of the present study was to investigate the effect of swimming exercise on elevated plus-maze (EPM)-associated memory deficit induced by intra-CA1 injection of scopolamine (a muscarinic acetylcholine receptor antagonist used to model Alzhe...

Mimo, R.; Sparacino, L.; Nicolosi, G.; D'Angelo, G.; Dall'Aglio, V.; Lestuzzi, C.; Pavan, D.; Cervesato, E.; Zanuttini, D., 1991: Quantification of mitral regurgitation: comparison between transthoracic and transesophageal color Doppler flow mapping. We reviewed transthoracic (TTE) and transesophageal (TEE) echocardiograms of 100 consecutive patients: 63 male, 37 female, mean age 50 years (range 16-83 years), 32 with neoplastic disease, 18 aortic disease, 28 mitral valve disease, and 22 with...

Bailey E.; Farmer P.B.; Campbell J.B., 1982: Determination of alkylated amino acids in hemo globin by capillary gas chromatography mass spectrometry. Analytical Proceedings 19(5): 239-241

Martin, V.; Ronde, P.; Funk, C.D., 1998: Cloning and functional expression of the mouse leukotriene B4 receptor. Molecular Biology of the Cell 9(SUPPL ): 215A, Nov

Haughton, J., 2000: A paradigm shift in healthcare. From disease management to patient-centered systems. M.D. Computing 17(4): 34-38

Lange, R.; Rupieper, N.; Ringert, R.H., 1988: Anesthesia in transurethral surgery. Transurethral resection (TUR) is a surgical operation generally performed in elderly patients, afflicted with polymorbidity and a high risk for anaesthesia. We studied 1351 transurethral resections during the years 1974-1984: 840 of these procedur...