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Preparation of ribo homo polymers of defined length

, : Preparation of ribo homo polymers of defined length. Analytical Biochemistry 95(1): 171-173

A method was developed for the preparation of defined-length homopolymers of long chain length in high yield. The method is based on the reaction of polynucleotide phosphorylase with 2 different ribonucleotide diphosphates and subsequent cleavage with a specific endo-RNase.

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Accession: 006173819

PMID: 495951

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Related references

Gabbay E.J.; Glaser R., 1970: Topography of nucleic acid helices in solutions comparative studies of the interactions of aliphatic di ammonium salts with double stranded and triple stranded deoxy ribo homo polymers hybrid homo polymers and ribo homo polymers. Biochimica et Biophysica Acta 224(1): 272-275

Aradi J.; Zsindely A.; Hauck M.; Tanko B., 1971: Preparation of ribo oligo nucleotides from homo polymers. Acta Biologica Debrecina 9: 13-17

Tan, E.S.; Krukenberg, K.A.; Mitchison, T.J., 2013: Large-scale preparation and characterization of poly(ADP-ribose) and defined length polymers. Poly(ADP-ribose) (pADPr) is a large, structurally complex polymer of repeating ADP-ribose units. It is biosynthesized from NAD<sup loc="post">+</sup> by poly(ADP-ribose) polymerases (PARPs) and degraded to ADP-ribose by poly(...

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Marquardt H.; Zedeck M.S.; Sternberg S.S.; Fleisher M.; Hamilton L., 1971: Poly ribo inosinic acid poly ribo cytidylic acid protective effect of gluco corticoids and lethal action of homo polymers. Naunyn-Schmiedebergs Archiv fuer Pharmakologie 269(2-4): 411

Skinner D.M.; Fowler R.F.; Bonnewell V., 1983: Sequence variations occur close to homo co polymers or homo polymers in a guanine and cytosine rich complex satellite dna. Federation Proceedings 42(7): ABSTRACT 2959

Schell P.L., 1971: Uptake of poly nucleotides by mouse ascites tumor cells part 10 ph dependent uptake of synthetic homo ribo polymers. Zeitschrift fuer Naturforschung Teil B Anorganische Chemie Organische Chemie Biochemie Biophysik Biologie 26(10): 1074-1076

Seliger H.; Zeh D.; Azuru G.; Chattopadhyaya J.B., 1983: 2 new and efficient routes to the preparation of oligo ribo nucleotides of defined sequence. Intermediates for oligoribonucleotide synthesis were prepared by a fast route using the tetraisopropyl-disiloxan-diyl group as a transient blocking group for the 3'- and 5'-hydroxyl functions. 2'-OH protection by tetrahydropyranyl-...

Vil'ner, L.M.; Brodskaya, L.M.; Kogan, E.M.; Timkovskii, A.L.; Sidorova, N.S., 1977: Anti viral and interferonogenic activity of synthetic complexes of homo poly ribo nucleotides and complexes of homo and hetero poly ribo nucleotides. Polynucleotide complexes were studied: poly(I) .cntdot. poly(C), poly(G) .cntdot. poly(C) and poly(G,I) .cntdot. poly(C). Poly(G) and poly(G,I) were synthesized using Escherichia coli polynucleotide phosphorylase. Antiviral and interferonogenic ac...

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