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Preparation of rnase free escherichia coli ribosomes active in initiation of protein biosynthesis


, : Preparation of rnase free escherichia coli ribosomes active in initiation of protein biosynthesis. Biokhimiya 46(9): 1640-1645

The level of contaminating RNase in the main components of the protein biosynthesis initiation system, the initiation factors and ribosomes of E. coli, was studied. The ribosomes are the major source of contaminating RNAase. A simple procedure for purification of ribosomes active in initiation including Sephadex G-200 gel filtration of unwashed ribosomes in a 1.5 M NH4Cl-containing buffer was developed.

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